GeneSpark™ Mouse PDX1 Promoter Primers (VS-0725-FY88)
CAT#: VS-0725-FY88
The Mouse PDX1 Promoter Primers offer a reliable tool for amplifying DNA isolated via chromatin immunoprecipitation (ChIP). This product features a pre-mixed set of forward and reverse PCR primers specifically targeting the promoter region of the mouse PDX1 (Pancreatic And Duodenal Homeobox 1) gene. PDX1 is a crucial transcription factor essential for pancreatic development and β-cell function, playing a key role in insulin production. These primers are optimized for use in SYBR® Green quantitative real-time PCR and are validated with Enzymatic Chromatin IP Kits and ChIP-validated antibodies. They are invaluable for studying gene regulation in pancreatic biology and diabetes research.
Specifications
- Application
- ChIP
- Application Notes
- In Chromatin Immunoprecipitation (ChIP) assays, primers are indispensable as precise detectors. Their core function is to facilitate the accurate identification and quantification of specific DNA sequences enriched by immunoprecipitation, thereby shedding light on crucial protein-DNA interactions.
- Workflow
- 1. Label PCR tubes/plates for duplicates, no-DNA control, and a 2% total input chromatin DNA serial dilution.
2. Add 2 μl ChIP DNA sample per well/tube.
3. Prepare and add 18 μl master PCR reaction mix (Nuclease-free H2O, Primers, SYBR® Green).
4. Run 40 cycles of PCR: 95°C for 3 min; then 95°C for 15 sec, primer-specific temp for 60 sec (repeated).
5. Analyze results with real-time PCR software.
- Size
- 0.5 mL
- Components
- These primers are supplied in nuclease-free water at a precise 5 μM concentration for each individual primer, ensuring accurate and contamination-free experimental setup.
- Biological Samples
- Mouse
- Storage
- Store at -20°C.
Target
- Full Name
- Pancreatic And Duodenal Homeobox 1
- Introduction
- The protein encoded by this gene is a transcriptional activator of several genes, including insulin, somatostatin, glucokinase, islet amyloid polypeptide, and glucose transporter type 2. The encoded nuclear protein is involved in the early development of the pancreas and plays a major role in glucose-dependent regulation of insulin gene expression. Defects in this gene are a cause of pancreatic agenesis, which can lead to early-onset insulin-dependent diabetes mellitus (IDDM), as well as maturity onset diabetes of the young type 4 (MODY4).
- Alternative Names
- Pancreatic And Duodenal Homeobox 1; Insulin Promoter Factor 1, Homeodomain Transcription Factor; Somatostatin-Transactivating Factor 1; Somatostatin Transcription Factor 1; Insulin Upstream Factor 1; Islet/Duodenum Homeobox-1; Glucose-Sensitive Factor; STF-1; IDX-1; IPF-1; IUF-1
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