Recombinant Rabbit Anti-FHL1 Antibody (clone R03-2H8) (CAT#: VS3-FY552)

Name: Recombinant Rabbit Anti-FHL1 Antibody (clone R03-2H8)
SKU: VS3-FY552
Rating: 5 (1 reviews)

This product is a recombinant rabbit antibody that recognizes FHL1. This antibody has been reported for use in Western Blot, Immunohistochemistry-Paraffin, Immunoprecipitation. The clone R03-2H8 is specific for human FHL1. The antigen is human fhl1 recombinant protein.

Custom Production

We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.

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Tested Data
Gene Expression
Datasheet
MSDS
COA

Figure 1. Recombinant Rabbit Anti-FHL1 Antibody (clone R03-2H8) in WB.

Figure 1. Recombinant Rabbit Anti-FHL1 Antibody (clone R03-2H8) in WB.

Western Blot analysis of FHL1 in Hela, U251 lysates using FHL1 Antibody.

IHC-P

Figure 2. Recombinant Rabbit Anti-FHL1 Antibody (clone R03-2H8) in IHC-P.

Figure 2. Recombinant Rabbit Anti-FHL1 Antibody (clone R03-2H8) in IHC-P.

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using an FHL1 antibody. High pressure and high temperature sodium citrate pH 6.0 for antigen retrieval.

Subcellular Location

Figure 1 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1040/if_selected.jpg

Normal Tissue

Figure 2 Kidney
(Cells in tubules Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/20817/46533_A_8_5.jpg

Figure 3 Skeletal muscle
(Myocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/20817/46533_B_2_6.jpg

Figure 4 Lymph node
(Germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/20817/46533_A_9_8.jpg

Figure 5 Cerebral cortex
(Endothelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuronal cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/20817/46533_B_9_5.jpg

Figure 6 Colon
(Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/20817/46533_A_7_3.jpg

RNA Expression

Figure 7 RNA cell line category: Cell line enhanced (BJ hTERT+, fHDF/TERT166)
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000022267-FHL1

Datasheet

MSDS

Certificate of Analysis Lookup
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number

Specifications

Immunogen
Human FHL1 recombinant protein.

Host Species
Rabbit

Type
Rabbit IgG

Specificity
Human FHL1

Species Reactivity
Human

Clone
R03-2H8

Applications
Western Blot, Immunohistochemistry-Paraffin, Immunoprecipitation

Conjugate
Unconjugated

MW
Calculated MW: 36 kDa; Observed MW: 36 kDa

Product Property

Purification
Affinity Purified

Purity
>95% as determined by SDS-PAGE

Buffer
50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% glycerol, 0.05% BSA

Preservative
0.01% Sodium azide

Storage
Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

Application Notes
Western Blot: 1/500-1/1000
Immunohistochemistry-Paraffin: 1/50-1/100
Immunoprecipitation: 1/20

Target

Alternative Names
Four And A Half LIM Domains 1; Skeletal Muscle LIM-Protein 1; LIM Protein SLIMMER; SLIM-1; FHL-1; SLIM1; SLIM; Four-And-A-Half Lin11, Isl-1 And Mec-3 Domains 1; Four And A Half LIM Domains Protein 1; Four-And-A-Half LIM Domains 1

Gene ID
2273

UniProt ID
Q13642

Secondary Antibody

Isotype Control

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

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IgG Antibody Products Rabbit Monoclonal Antibody Products IHC Kit and Antibody Products: Precision Tools for Immunohistochemistry

CAT	Product Name	Application	Type
MOB-0741CT	Recombinant Mouse anti-Human FHL1 Monoclonal antibody (EML2148)	WB
ZG-440C	Human Anti-FHL1 Recombinant Antibody (ZG-440C)	ELISA, IP	Human IgG1, κ
VS3-WK950	Rabbit Anti-FHL1 Recombinant Antibody (VS3-WK950)	WB, IHC, IF	Rabbit IgG
VS13-YC397	Rabbit Anti-FHL1 Recombinant Antibody (VS13-YC397)	WB, IHC-P, IP	Rabbit IgG

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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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