Recombinant Mouse Anti-KRT14/KRT16/KRT5/KRT6A/KRT8 Antibody (VS4-CJ4) (CAT#: VS4-CJ4)

• Immunogen
• Synthesized peptide derived from human Cytokeratin Pan

• Host Species
• Mouse

• Type
• Mouse IgG2b, κ

• Specificity
• Human KRT14/KRT16/KRT5/KRT6A/KRT8

• Species Reactivity
• Human

• Applications
• ELISA, IHC

• Conjugate
• Unconjugated

• Purification
• Immunogen affinity chromatography

• Purity
• >95% as determined by SDS-PAGE

• Format
• Liquid

• Buffer
• 50% Glycerol, 0.5% BSA, PBS

• Preservative
• 0.02% Sodium Azide

• Storage
• Store at -20°C or -80°C upon receipt. Avoid repeated freeze.

• Application Notes
• This antibody has been tested for use in ELISA, Immunohistochemistry (1:20-1:200).

• Alternative Names
• CARD2; CK-4; CK-6C; CK-6E; CK-8; CK14; CK16; CK4; CK5; CK6A; CK6C; CK6D; CK8; CYK4; CYK8; DDD; DDD1; EBS2; EBS3; EBS4; FNEPPK; K14; K16; K1CP; K2C8; K4; K5; K6A; K6C; K6D; K8; KO; KRT14; KRT16; KRT16A; KRT5; KRT5A; KRT6A; KRT6C; KRT6D; KRT8; NEPPK; NFJ; PC1; PC3; WSN1; 58 kDa cytokeratin; CK-14; CK-16; CK-5; CK-6A; CK-6D; CK-8; cytokeratin 14; cytokeratin 16; cytokeratin 4; cytokeratin 6A; cytokeratin 6C; cytokeratin 6D; Cytokeratin-14; Cytokeratin-16; Cytokeratin-5; Cytokeratin-6A; cytokeratin-6C; Cytokeratin-6D; cytokeratin-6E; Cytokeratin-8; epidermolysis bullosa simplex 2 Dowling-Meara/Kobner/Weber-Cockayne types; focal non-epidermolytic palmoplantar keratoderma; Hom s 5; K14; K16; K5; K6A; K8; kamp-keratin derived antimicrobial peptide; KDAMP; keratin 14, type I; keratin 16, type I; keratin 4, type II; keratin 5 (epidermolysis bullosa simplex, Dowling-Meara/Kobner/Weber-Cockayne types); keratin 5, type II; keratin 6A, type II; keratin 6A, type II; keratin 8, type II; keratin K6h; keratin, epidermal type II, K6A; Keratin, type I cytoskeletal 14; Keratin, type I cytoskeletal 16; Keratin, type II cytoskeletal 5; Keratin, type II cytoskeletal 6A; Keratin, type II cytoskeletal 8; Keratin-14; Keratin-16; Keratin-5; Keratin-6A; keratin-6C; Keratin-8; pankeratin; type-II keratin Kb12; type-II keratin Kb4; Type-II keratin Kb5; Type-II keratin Kb6; Type-II keratin Kb8

• Gene ID
• 3861; 3868; 3852; 3853; 3856

• UniProt ID
• P02533; P08779; P13647; P02538; P05787

• Long Name
• Keratin 14/16/5/6A/8

• Sequence Similarities
• Belongs to the intermediate filament family.

• Cellular Localization
• Cytoplasm, Intermediate filament, Keratin, Nucleus

• Post Translation Modifications
• A disulfide bond is formed between rather than within filaments and promotes the formation of a keratin filament cage around the nucleus.
  Ubiquitinated by the BCR(KLHL24) E3 ubiquitin ligase complex.

• Protein Refseq
• NP_000517.2

• Function
• The nonhelical tail domain is involved in promoting KRT5-KRT14 filaments to self-organize into large bundles and enhances the mechanical properties involved in resilience of keratin intermediate filaments in vitro.

Isotype Control

• MOB-203CQ
• Mouse IgG2b, kappa Isotype Control Antibody

• MOB-212YJL
• Mouse IgG2b kappa Isotype Control Antibody (Mopc21)

Recommended Dilution Buffer

• MRO-0253YJ
• Reconstitution Buffer PBS