Recombinant Mouse Anti-MCM9 Antibody (clone 5H8E10) (CAT#: VS4-CJ32)

• Immunogen
• Recombinant Human DNA helicase MCM9 protein (1aa-391aa)

• Host Species
• Mouse

• Type
• Mouse IgG1

• Specificity
• Human MCM9

• Species Reactivity
• Human

• Clone
• 5H8E10

• Applications
• ELISA, WB, IHC

• Conjugate
• Unconjugated

• Purification
• Protein G purified

• Purity
• >95% as determined by SDS-PAGE

• Format
• Liquid

• Buffer
• 50% Glycerol, 0.01M PBS, pH 7.4.

• Preservative
• 0.03% Proclin 300

• Storage
• Store at -20°C or -80°C upon receipt. Avoid repeated freeze.

• Application Notes
• This antibody has been tested for use in ELISA, Western Blot (1:1000-1:5000), Immunohistochemistry (1:50-1:200).

• Alternative Names
• MCM9 antibody; MCMDC1DNA helicase MCM9; hMCM9; EC 3.6.4.12; Mini-chromosome maintenance deficient domain-containing protein 1; Minichromosome maintenance 9; ODG4; MCMDC1; C6orf61; dJ329L24.1; dJ329L24.3

• Gene ID
• 254394

• UniProt ID
• Q9NXL9

• Long Name
• Minichromosome maintenance 9 homologous recombination repair factor

• Sequence Similarities
• Belongs to the MCM family.

• Cellular Localization
• Chromosome, Nucleus

• Protein Refseq
• NP_060166.2; NP_694987.1

• Function
• Component of the MCM8-MCM9 complex, a complex involved in the repair of double-stranded DNA breaks (DBSs) and DNA interstrand cross-links (ICLs) by homologous recombination (HR) (PubMed:23401855).
  Required for DNA resection by the MRE11-RAD50-NBN/NBS1 (MRN) complex by recruiting the MRN complex to the repair site and by promoting the complex nuclease activity (PubMed:26215093).
  Probably by regulating the localization of the MRN complex, indirectly regulates the recruitment of downstream effector RAD51 to DNA damage sites including DBSs and ICLs (PubMed:23401855).
  Acts as a helicase in DNA mismatch repair (MMR) following DNA replication errors to unwind the mismatch containing DNA strand (PubMed:26300262).
  In addition, recruits MLH1, a component of the MMR complex, to chromatin (PubMed:26300262).
  The MCM8-MCM9 complex is dispensable for DNA replication and S phase progression (PubMed:23401855).
  Probably by regulating HR, plays a key role during gametogenesis (By similarity).

Isotype Control

• MOB-065CQ
• Mouse IgG1 Isotype Control Antibody

Secondary Antibody

• 2RD-A1562-YJ
• Rabbit Anti-Mouse IgG1 Secondary antibody (Clone RM106)

• 2RD-A1460-YJ
• Goat Anti-Mouse IgG1 Secondary antibody

Recommended Dilution Buffer

• MRO-0253YJ
• Reconstitution Buffer PBS