10-FnIII scaffold protein anti-Human TNFα (CAT#: SFN-L026)
The tenth fibronectin type III domain (10Fn3) that bound TNF-a was isolated from the library using mRNA Display technology. The fibronectin type III domain (FN3) is a small autonomous folding unit which occurs in many animal proteins involving in ligand binding. The β-sandwich structure of FN3 closely resembles that of immunoglobulin domains. After affinity maturation, 10Fn3 variants were identified having affinities (KD=20 pM) for TNF-a as determined by biosensor technology. It's potential to be used in diagnostic, research and therapeutic applications.

(Germinal center cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/78469/168112_A_4_8.jpg

(Cell lines ordered by descending RNA expression order.)
* https://v21.proteinatlas.org/ENSG00000232810-TNF

(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000232810-TNF
Specifications
- Scaffold Name
- 10-FnIII
- Origin
- Fibronectin
- Core Structure
- β-sandwich of 7 β-strands
- Variable Regions
- 2–3 loops
- Target
- TNFα
- Species Reactivity
- Human
- Expression Host
- E. coli
- Affinity Constant
- 20 pM
- Applications
- ELISA; immunohistochemistry; Microscopy; Flow Cytometer; Western Blotting; Functional Study
Target
- Alternative Names
- Fibronectin; 10-FnIII; DIF; TNFA; TNFSF2; TNLG1F; TNF-alpha
- Gene ID
- 7124
- UniProt ID
- P01375
Related Resources
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "TNF"
Select a product category from the dropdown menu below to view related products.
CAT | Product Name | Application | Type |
---|---|---|---|
MOB-1076z | Mouse Anti-TNF Recombinant Antibody (clone 18C3) | ELISA, FC, FuncS | Mouse IgG1, κ |
PABL-349 | Human Anti-TNF Recombinant Antibody (clone A2) | WB, ELISA, FuncS | Human IgG |
HPAB-0203-WJ | Rat Anti-TNF Recombinant Antibody (HPAB-0203-WJ) | Neut, ELISA | Rat IgG |
HPAB-M0219-YC | Rabbit Anti-TNF Recombinant Antibody (HPAB-M0219-YC) | ELISA, Neut, Inhib, FuncS | Rabbit IgG |
HPAB-M0220-YC | Rabbit Anti-TNF Recombinant Antibody (HPAB-M0220-YC) | ELISA, Neut, Inhib, FuncS | Rabbit IgG |
Customer Reviews and Q&As
There are currently no Customer reviews or questions for SFN-L026. Click the button above to contact us or submit your feedback about this product.
Popular products with customers
Application: IF, IP, Neut, FuncS, ELISA, FC, WB
Application: IF, IP, Neut, FuncS, ELISA, FC, WB
Application: ELISA, FC, IP, FuncS, IF, Neut, ICC
Application: ELISA, FuncS, IHC, IF, FC, ADCC
Application: ELISA
Application: Neut, FC, IHC-Fr, IP, BA
Application: ELISA, FuncS, Neut, IF, WB, EM, Inhib, IHC
Application: ELISA, Inhib, FuncS
Application: ELISA, WB
For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.