Human Anti-TNF (clone BPC1500) scFv-Fc Chimera (CAT#: VS-0125-FY32)

• Host Species
• Human

• Type
• Human IgG1, scFv-Fc

• Species Reactivity
• Human

• Clone
• BPC1500

• Applications
• ELISA, Neutralization

• Purity
• >95% as determined by SDS-PAGE

• Format
• Liquid

• Concentration
• Please refer to the vial label for the specific concentration.

• Buffer
• PBS

• Preservative
• No preservatives

• Stabilizer
• No Stabilizers

• Sterility
• 0.2 μM filtered

• Storage
• Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

• Shipping
• Ice packs

• Application Notes
• The function of this scFv-Fc fusion protein is to possess both specific binding capacity to the synaptic membrane and neutralization ability against TNFα. Through this combination, the goal is to directly deliver anti-TNF effects at the site of inflammation in the joints, while reducing systemic TNF binding activity, potentially decreasing systemic side effects and enhancing therapeutic efficacy.

• Alternative Names
• Tumor Necrosis Factor; Tumor Necrosis Factor Ligand Superfamily Member 2; Cachectin; TNF-Alpha; TNFSF2; TNF-A; TNFA; Tumor Necrosis Factor (TNF Superfamily, Member 2); Tumor Necrosis Factor Ligand 1F

• Gene ID
• 7124

• UniProt ID
• P01375

• Sequence Similarities
• Belongs to the tumor necrosis factor family.

• Cellular Localization
• Cell membrane, Membrane, Secreted

• Post Translation Modifications
• The soluble form derives from the membrane form by proteolytic processing. The membrane-bound form is further proteolytically processed by SPPL2A or SPPL2B through regulated intramembrane proteolysis producing TNF intracellular domains (ICD1 and ICD2) released in the cytosol and TNF C-domain 1 and C-domain 2 secreted into the extracellular space.
  The membrane form, but not the soluble form, is phosphorylated on serine residues. Dephosphorylation of the membrane form occurs by binding to soluble TNFRSF1A/TNFR1.
  O-glycosylated; glycans contain galactose, N-acetylgalactosamine and N-acetylneuraminic acid.
  Tumor necrosis factor, soluble form: The soluble form is demyristoylated at Lys-19 and Lys-20 by SIRT6, promoting its secretion.

• Protein Refseq
• NP_000585.2

• Function
• Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. Impairs regulatory T-cells (Treg) function in individuals with rheumatoid arthritis via FOXP3 dephosphorylation. Up-regulates the expression of protein phosphatase 1 (PP1), which dephosphorylates the key 'Ser-418' residue of FOXP3, thereby inactivating FOXP3 and rendering Treg cells functionally defective.
  Key mediator of cell death in the anticancer action of BCG-stimulated neutrophils in combination with DIABLO/SMAC mimetic in the RT4v6 bladder cancer cell line.
  Induces insulin resistance in adipocytes via inhibition of insulin-induced IRS1 tyrosine phosphorylation and insulin-induced glucose uptake. Induces GKAP42 protein degradation in adipocytes which is partially responsible for TNF-induced insulin resistance (By similarity).
  Plays a role in angiogenesis by inducing VEGF production synergistically with IL1B and IL6.
  The TNF intracellular domain (ICD) form induces IL12 production in dendritic cells.

Secondary Antibody

• 2ND-029CQ
• Goat Anti-Human IgG Fc Antibody

Isotype Control

• MOB-200CQ
• Human IgG Fc Isotype Control Antibody

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