Chicken Anti-Tumor Necrosis Factor-α Polyclonal IgY (CAT#: BRD-0609MZ)

• Excellent for ELISA
  Having tested various antibodies, the Chicken Anti-TNF-α Polyclonal IgY stands out for its low cross-reactivity and high affinity. Our sandwich ELISA results are now much more sensitive and accurate.
• High specificity and reduced background
  I highly recommend the Chicken Anti-Tumor Necrosis Factor-α Polyclonal IgY for anyone working with TNF-α. Its high specificity and reduced background have significantly improved the quality of our data.
• Reliable in WB and ELISA
  This antibody has become a staple in our lab for TNF-α detection. Its broad epitope recognition ensures consistent detection across different assay platforms, from ELISA to Western blots.

1. Q 1: In terms of cross-reactivity with human TNF-α, how does the chicken anti-tumor necrosis factor-α polyclonal IgY compare to mammalian antibodies?

   A: Chicken ANF-α Polyclonal IgY has fewer cross-reactivity than mammalian antibodies because of evolutionary differences between chickens and mammals. The chicken IgY method detects human TNF-α with high specificity and negligible background noise.

2. Q 2: How should the Chicken Anti-TNF-α Polyclonal IgY be preserved to get the longest possible shelf life?

   A: Anti-Tumor Necrosis Factor-α Polyclonal IgY for chickens should be stored at -20°C. It can be stored for a month or less at 4°C for short-term use. To preserve the integrity of the antibody, avoid doing freeze-thaw cycles too often.

3. Q 3: Which secondary antibodies work best together for detection in Western blot experiments with it?

   A: Secondary antibodies produced against chicken IgY coupled with HRP or alkaline phosphatase are effective in detecting Chicken Anti-TNF-α Polyclonal IgY in Western blot experiments, resulting in clear and specific signal detection.

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