Recombinant Mouse Anti-B. germanica Bla g 2 Antibody Fab Fragment (7C11) (CAT#: PFBC-013)

Figure 1 Antibody binding to rBla g 2 presented by mAb 7C11 or 4C3 using multiplex array.

A) Anti-Bla g 2 polyclonal antibody bound to rBla g 2 (mean ± standard deviation, n = 5 experiments, p = 0.059). B) IgE from sera of cockroach allergic patients bound to rBla g 2. The allergen was presented by either mAb 7C11 (gray) or 4C3 (black). The first and second row of numbers under the X-axis are ratio of IgE bound to Bla g 2 presented by mAb 7C11 versus mAb 4C3 (100%) and serum number, respectively. MFI are Median Fluorescence Intensity Units.

Glesner, J., Wünschmann, S., Li, M., Gustchina, A., Wlodawer, A., Himly, M., ... & Pomés, A. (2011). Mechanisms of allergen-antibody interaction of cockroach allergen Bla g 2 with monoclonal antibodies that inhibit IgE antibody binding. PloS one, 6(7), e22223.

Figure 2 Dose-response curves of Bla g 2 and epitope mutants by ELISA.

A) Binding of the anti-Bla g 2 polyclonal antibody to mAb 7C11 epitope mutants. B) Binding of the anti-Bla g 2 polyclonal antibody to mAb 4C3 epitope mutants. C) Binding of mAb 7C11 to mAb 7C11 epitope mutants, D) Binding of mAb 4C3 to mAb 4C3 epitope mutants. Data are mean ± standard deviation of duplicates from one representative experiment out of two performed for each panel. Legends in panels C and D also apply to panels A and B, respectively.

Glesner, J., Wünschmann, S., Li, M., Gustchina, A., Wlodawer, A., Himly, M., ... & Pomés, A. (2011). Mechanisms of allergen-antibody interaction of cockroach allergen Bla g 2 with monoclonal antibodies that inhibit IgE antibody binding. PloS one, 6(7), e22223.