Recombinant Mouse Anti-B. germanica Bla g 2 Antibody Fab Fragment (7C11) (CAT#: PFBC-013)
Recombinant Mouse Antibody (7C11) Fab Fragment is specific to B. germanica Bla g 2, expressed in Chinese Hamster Ovary cells (CHO).
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
Figure 1 Antibody binding to rBla g 2 presented by mAb 7C11 or 4C3 using multiplex array.
A) Anti-Bla g 2 polyclonal antibody bound to rBla g 2 (mean ± standard deviation, n = 5 experiments, p = 0.059). B) IgE from sera of cockroach allergic patients bound to rBla g 2. The allergen was presented by either mAb 7C11 (gray) or 4C3 (black). The first and second row of numbers under the X-axis are ratio of IgE bound to Bla g 2 presented by mAb 7C11 versus mAb 4C3 (100%) and serum number, respectively. MFI are Median Fluorescence Intensity Units.
Glesner, J., Wünschmann, S., Li, M., Gustchina, A., Wlodawer, A., Himly, M., ... & Pomés, A. (2011). Mechanisms of allergen-antibody interaction of cockroach allergen Bla g 2 with monoclonal antibodies that inhibit IgE antibody binding. PloS one, 6(7), e22223.
Figure 2 Dose-response curves of Bla g 2 and epitope mutants by ELISA.
A) Binding of the anti-Bla g 2 polyclonal antibody to mAb 7C11 epitope mutants. B) Binding of the anti-Bla g 2 polyclonal antibody to mAb 4C3 epitope mutants. C) Binding of mAb 7C11 to mAb 7C11 epitope mutants, D) Binding of mAb 4C3 to mAb 4C3 epitope mutants. Data are mean ± standard deviation of duplicates from one representative experiment out of two performed for each panel. Legends in panels C and D also apply to panels A and B, respectively.
Glesner, J., Wünschmann, S., Li, M., Gustchina, A., Wlodawer, A., Himly, M., ... & Pomés, A. (2011). Mechanisms of allergen-antibody interaction of cockroach allergen Bla g 2 with monoclonal antibodies that inhibit IgE antibody binding. PloS one, 6(7), e22223.
Specifications
- Immunogen
- B. germanica Bla g 2
- Host Species
- Mouse
- Derivation
- Mouse
- Type
- IgG
- Specificity
- Tested positive against native B. germanica Bla g 2
- Species Reactivity
- B. germanica
- Clone
- 7C11
- Applications
- ELISA Assay-Dependent
Product Property
- Purity
- >95% by SDS-PAGE and HPLC analysis
- Storage
- Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing of samples.
Applications
- Application Notes
- The antibody Fab fragment 7C11 has been reported in application of ELISA. It's recommended that the optimal antibody concentration, dilution, incubition time etc. are best to be carefully titrated in specific assays.
Target
- Alternative Names
- Bla g 2
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "Clone 7C11"
See other products for "Bla g 2"
Select a product category from the dropdown menu below to view related products.
CAT | Product Name | Application | Type |
---|---|---|---|
MOB-112 | Recombinant Anti-Bla g 2 Antibody | WB, FC, FuncS | IgG |
MHH-112 | Recombinant Human Anti-Bla g 2 Antibody | RIA, WB, FuncS | IgG |
PABC-013 | Recombinant Mouse Anti-B. germanica Bla g 2 Antibody (7C11) | ELISA | IgG |
HPAB-S0180-YC | Mouse Anti-Bla g 2 Recombinant Antibody (clone 7C11) | ELISA | Mouse IgG |
VS-0322-LC62 | Rabbit Anti-Bla g 2 Recombinant Antibody (clone 4C3) | ELISA |
Customer Reviews and Q&As
Thorough Datasheet
Outstanding Flexibility
Reliable Outcomes
Popular products with customers
Application: FC, Cyt, Stim, PP, Agonist
Application: WB, FuncS, IF, Neut, ELISA, FC, IP
Application: WB, IF, IP, Neut, FuncS, ELISA, FC
Application: ELISA, IHC
Application: FC, IP, ELISA, Neut, FuncS, IF, WB
Application: WB, Neut, ELISA, IF, IP, FuncS, FC
Application: IB, ELISA, FC, FuncS
Application: Neut, Inhib, FC, ELISA
Application: ELISA, PD, Activ, PK, Stim
For Research Use Only. Not For Clinical Use.
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.