Mouse Anti-Bla g 2 Recombinant Antibody (clone 7C11) (CAT#: HPAB-S0180-YC)
The crystal structure of a 1:1 complex between the German cockroach allergen Bla g 2 and the Fab′ fragment of a monoclonal antibody 7C11 was solved at 2.8-Å resolution. Bla g 2 binds to the antibody through four loops that include residues 60-70, 83-86, 98-100, and 129-132. The epitope for mAb 7C11 is important surface determinants overlapping with IgE antibody binding sites and inhibit IgE antibody binding.
Specific Inquiry
Figure 1 Overlap of 7C11 mAb and IgE antibody epitopes.
Increasing concentrations of mAb 7C11 showed dose dependent inhibition of IgE antibody binding of up to 40%.
Glesner, J., Wünschmann, S., Li, M., Gustchina, A., Wlodawer, A., Himly, M., ... & Pomés, A. (2011). Mechanisms of allergen-antibody interaction of cockroach allergen Bla g 2 with monoclonal antibodies that inhibit IgE antibody binding. PloS one, 6(7), e22223.
Figure 2 Antibody binding to rBla g 2 presented by mAb 7C11 or 4C3 using multiplex array.
Polyclonal anti-Bla g 2 antibody binding to the allergen monomer was very similar for the allergen presented by either mAb 7C11 or 4C3.
Glesner, J., Wünschmann, S., Li, M., Gustchina, A., Wlodawer, A., Himly, M., ... & Pomés, A. (2011). Mechanisms of allergen-antibody interaction of cockroach allergen Bla g 2 with monoclonal antibodies that inhibit IgE antibody binding. PloS one, 6(7), e22223.
ELISA
Figure 3 Inhibition of biotinylated mAb 7C11 binding to rBla g 2 by allergen mutants using ELISA.
The order of potency of the mutants for inhibiting mAb 7C11 binding to Bla g 2 was: natural Bla g 2> Bla g 2-N93Q> R83A-E86A> K65A> D68aA> K132A.
Glesner, J., Wünschmann, S., Li, M., Gustchina, A., Wlodawer, A., Himly, M., ... & Pomés, A. (2011). Mechanisms of allergen-antibody interaction of cockroach allergen Bla g 2 with monoclonal antibodies that inhibit IgE antibody binding. PloS one, 6(7), e22223.
ELISA
Figure 4 Specificity and affinity for phage binding to mAb 7C11.
The two clones bound to 7C11 with similar affinity, while the control phage did not bind.
Tiwari, R., Negi, S. S., Braun, B., Braun, W., Pomés, A., Chapman, M. D., ... & Midoro-Horiuti, T. (2012). Validation of a phage display and computational algorithm by mapping a conformational epitope of Bla g 2. International archives of allergy and immunology, 157(4), 323-330.
ELISA
Figure 5 Phage peptide inhibition of Bla g 2 binding to mAb 7C11.
The ability of both phage clones to inhibit the binding of rBla g 2 to 7C11 was similar, confirming that these two peptides bind to mAb 7C11 with similar specificity and affinities.
Tiwari, R., Negi, S. S., Braun, B., Braun, W., Pomés, A., Chapman, M. D., ... & Midoro-Horiuti, T. (2012). Validation of a phage display and computational algorithm by mapping a conformational epitope of Bla g 2. International archives of allergy and immunology, 157(4), 323-330.
Specifications
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Cockroach Bla g 2
- Species Reactivity
- Cockroach
- Clone
- 7C11
- Applications
- ELISA
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Buffer
- PBS
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
- Alternative Names
- Cockroach Allergen Bla g 2
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-112 | Recombinant Anti-Bla g 2 Antibody | WB, FC, FuncS | IgG |
| MHH-112 | Recombinant Human Anti-Bla g 2 Antibody | RIA, WB, FuncS | IgG |
| PABC-013 | Recombinant Mouse Anti-B. germanica Bla g 2 Antibody (7C11) | ELISA | IgG |
| VS-0322-LC62 | Rabbit Anti-Bla g 2 Recombinant Antibody (clone 4C3) | ELISA |
Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-112-F(E) | Recombinant Anti-Bla g 2 Antibody Fab Fragment | ELISA, Nert, FuncS | Fab |
| MHH-112-F(E) | Recombinant Human Anti-Bla g 2 Antibody Fab Fragment | ELISA, WB, IF, FuncS | Fab |
| PFBC-013 | Recombinant Mouse Anti-B. germanica Bla g 2 Antibody Fab Fragment (7C11) | ELISA | IgG |
| HPAB-S0180-YC-F(E) | Mouse Anti-Bla g 2 Recombinant Antibody (clone 7C11); Fab Fragment | ELISA | Mouse Fab |
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-112-S(P) | Recombinant Anti-Bla g 2 Antibody scFv Fragment | ELISA, WB, FC, FuncS | scFv |
| MHH-112-S(P) | Recombinant Human Anti-Bla g 2 Antibody scFv Fragment | ELISA, WB, IP, FuncS | scFv |
| PSBC-013 | Recombinant Mouse Anti-B. germanica Bla g 2 Antibody scFv Fragment (7C11) | ELISA | scFv |
| HPAB-S0180-YC-S(P) | Mouse Anti-Bla g 2 Recombinant Antibody (clone 7C11); scFv Fragment | ELISA | Mouse scFv |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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