Anti-CD3 Antibody-Cytokine Fusion Protein, TNF-IgG (OKT3) (CAT#: ACFP-SH129)
This antibody-cytokine fusion protein was achieved by conjugating/fusing the Anti-CD3 IgG to TNF. It was expressed in CHO and purified with affinity chromatography. The immunocytokine retains the ability to bind the CD3 as well as the biological activity of TNF. TNF-FuP provided a weak stimulus for lymphocyte proliferation, but it had no effect on LAK cells. However, it supported activation of Mfs and, to a minor extent, of CTLs. This immunocytokine was designed for treating CD30-positive lymphoma.
Specific Inquiry
Subcellular Location and Protein Expression
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line JURKAT
(Immunofluorescent staining of human cell line JURKAT shows localization to plasma membrane & endoplasmic reticulum.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/40957/1970_E5_1_selected.jpg
(Immunofluorescent staining of human cell line JURKAT shows localization to plasma membrane & endoplasmic reticulum.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/40957/1970_E5_1_selected.jpg
Figure 2 Lymph node
(Non-germinal center cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/72864/157655_A_9_8.jpg
(Non-germinal center cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/72864/157655_A_9_8.jpg
Figure 3 RNA cell line category: Group enriched (JURKAT, MOLT-4)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000198851-CD3E
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000198851-CD3E
Specifications
- Target
- CD3
- Molecule Class
- TNF-IgGa
- Species Reactivity
- Human
- Expression Host
- CHO
- Clone
- OKT3
- Related Disease
- CD30-positive lymphoma
- Cytokine 1
- TNF
- Cytokines 1 Description
- Tumor necrosis factor (TNF), formerly known as TNFα or TNF alpha, is the best-known member of this class. TNF is a monocyte-derived cytotoxin that has been implicated in tumor regression, septic shock, and cachexia. The protein is synthesized as a prohormone with an unusually long and atypical signal sequence, which is absent from the mature secreted cytokine. A short hydrophobic stretch of amino acids serves to anchor the prohormone in lipid bilayers. Both the mature protein and a partially processed form of the hormone can be secreted after cleavage of the propeptide.
- Cytokine 1 Species
- Human
- Applications
- This product can be used therapeutically to deliver biologically active ligands to a desired tissue.
Target
- Alternative Names
- CD3E; CD3e molecule; epsilon (CD3-TCR complex); T3E; TCRE; IMD18; T-cell surface glycoprotein CD3 epsilon chain; CD3-epsilon; T-cell surface antigen T3/Leu-4 epsilon chain; CD3e antigen; epsilon polypeptide (TiT3 complex); T-cell antigen receptor complex; epsilon subunit of T3
- Gene ID
- 916
- UniProt ID
- P07766
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Rat Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-H28 | Anti-Human CD3E Recombinant Antibody (Ertumaxomab) | ELISA, FC, IP, FuncS, IF, Neut, ICC | IgG2a / G2b |
Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| DrMAB-988 | Mouse Anti-CD3E Recombinant Antibody (clone UCHT1) | FC, IHC, WB, FuncS, IF | Mouse IgG1 F(ab')2 |
| DrMAB-989 | Mouse Anti-CD3E Recombinant Antibody (clone UCHT1); Fab Fragment | FC, Cyt, Stim, PP, Agonist | Mouse Fab |
| HPAB-0263-YJ-F(E) | Rabbit Anti-CD3E Recombinant Antibody (clone 645); Fab Fragment | ELISA, FC, FuncS | Rabbit Fab |
| HPAB-0347-YJ-F(E) | Rat Anti-CD3E Recombinant Antibody (clone 1Ab); Fab Fragment | FC, WB, Inhib | Rat Fab |
| HPAB-0385-FY-F(E) | Human Anti-CD3E Recombinant Antibody; Fab Fragment (HPAB-0385-FY-F(E)) | Activ | Human Fab |
Humanized Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-714 | Anti-CD3E Recombinant Antibody (Visilizumab) | FuncS, IF, Neut, ELISA, FC, IP, ICC | IgG2 |
| TAB-H52 | Anti-Human CD3E Recombinant Antibody (Otelixizumab) | FC, IP, ELISA, Neut, FuncS, IF, WB | IgG1 - lambda |
| TAB-420CQ | Anti-Human CD3E Recombinant Antibody (Visilizumab) | ELISA, IHC, FC, IP, IF, FuncS | IgG2, κ |
Human Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-245 | Anti-Human CD3 epsilon Recombinant Antibody (Foralumab) | WB, ELISA, FC, IP, FuncS, IF, Neut | IgG1 - kappa |
| TAB-124CL | Human Anti-CD3E Recombinant Antibody (TAB-124CL) | ELISA | Human IgG |
Mouse Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-019 | Anti-Human CD3 Recombinant Antibody (Muromonab-CD3) | FC, IP, ELISA, Neut, FuncS, IF, WB | IgG2a - kappa |
| PABX-032-S (P) | Recombinant Grey hamster Anti-CD3E Antibody scFv Fragment (2C11) | WB, ELISA, FuncS | scFv |
| PABX-033-S (P) | Recombinant Grey hamster Anti-CD3E Antibody scFv Fragment (17A2) | WB, ELISA, FuncS | scFv |
Immunotoxin
| CAT | Product Name | Application | Type |
|---|---|---|---|
| AGTO-G010E | Anti-CD3E immunotoxin UCHT1 (scFv)-PE | Cytotoxicity assay, Function study | |
| AGTO-G010D | Anti-CD3E immunotoxin UCHT1 (scFv)-DT | Cytotoxicity assay, Function study | |
| AGTO-L049E | anti-CD3E immunotoxin UCHT1 (IgG)-PE | Cytotoxicity assay, Functional assay | |
| AGTO-L049D | anti-CD3E immunotoxin UCHT1 (IgG)-DT | Cytotoxicity assay, Functional assay | |
| AGTO-L049R | anti-CD3E immunotoxin UCHT1 (IgG)-RTA | Cytotoxicity assay, Functional assay |
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-600NL | Recombinant Anti-mouse CD3e Antibody. | IP, IHC-Fr, FuncS, ICC, FACS | |
| PABL-648 | Mouse Anti-CD3E Recombinant Antibody (clone 2C11) | WB, IHC, IF, FuncS | Mouse IgG |
| HPAB-0263-YJ | Rabbit Anti-CD3E Recombinant Antibody (clone 645) | ELISA, FC, FuncS | Rabbit IgG1 |
| HPAB-A1283-YJ | Hamster Anti-CD3E Recombinant Antibody (clone 145.2c11) | ELISA, WB, IP, FC, FuncS, Inhib | Hamster IgG |
| VS4-WK82 | Mouse Anti-CD3E Recombinant Antibody (VS4-WK82) | ELISA, IF, FC | Mouse IgG2a |
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PSBL-643 | Mouse Anti-CD3E Recombinant Antibody (clone 2C11); scFv Fragment | WB, IHC, IF, FuncS | Mouse scFv |
| FAMAB-0049-YC-S(P) | Hamster Anti-CD3E Recombinant Antibody (clone 145-2C11); scFv Fragment | FC, FuncS | Hamster scFv |
| FAMAB-0052-YC-S(P) | Hamster Anti-CD3E Recombinant Antibody (clone 500A2); scFv Fragment | ELISA | Hamster scFv |
| HPAB-0263-YJ-S(P) | Rabbit Anti-CD3E Recombinant Antibody (clone 645); scFv Fragment | ELISA, FC, FuncS | Rabbit scFv |
| HPAB-0347-YJ-S(P) | Rat Anti-CD3E Recombinant Antibody (clone 1Ab); scFv Fragment | FC, WB, Inhib | Rat scFv |
Chimeric Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABX-032 | Recombinant Grey hamster Anti-CD3E Antibody (2C11) | WB, ELISA, FuncS, IP | IgG |
| PABX-033 | Recombinant Grey hamster Anti-CD3E Antibody (17A2) | WB, ELISA, FuncS | IgG |
| PABX-032-F (E) | Recombinant Grey hamster Anti-CD3E Antibody Fab Fragment (2C11) | WB, ELISA, FuncS | Fab |
| PABX-033-F (E) | Recombinant Grey hamster Anti-CD3E Antibody Fab Fragment (17A2) | WB, ELISA, FuncS | Fab |
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0558 | Hi-Affi™ Rabbit Anti-CD3E Recombinant Antibody (clone DS558AB) | IHC-P | Rabbit IgG |
| MOR-0058-FY | Rabbit Anti-CD3E Recombinant Antibody (clone AFY0029) | FC, ICC, IF, IHC-P, WB | Rabbit IgG |
ADCC Enhanced Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| AFC-TAB-245 | Afuco™ Anti-CD3E ADCC Recombinant Antibody (Foralumab), ADCC Enhanced | ELISA, FC, IP, FuncS, IF | ADCC enhanced antibody |
| AFC-TAB-H52 | Afuco™ Anti-CD3E ADCC Recombinant Antibody (Otelixizumab), ADCC Enhanced | FC, IP, ELISA, Neut, FuncS, IF | ADCC enhanced antibody |
| AFC-TAB-714 | Afuco™ Anti-CD3E ADCC Recombinant Antibody (Visilizumab), ADCC Enhanced | FuncS, IF, Neut, ELISA, FC, IP | ADCC enhanced antibody |
| AFC-TAB-019 | Afuco™ Anti-CD3E ADCC Recombinant Antibody (Muromonab-Cd3), ADCC Enhanced | FC, IP, ELISA, Neut, FuncS, IF | ADCC enhanced antibody |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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