Anti-Human CLDN1 Recombinant Antibody (5F2) (CAT#: TAB-481LC)

Recombinant monoclonal antibody to CLDN1. 5F2 is a mouse monoclonal antibody that can be potentially used in the treatment of hepatitis C.


Specific Inquiry
  • Size:
  • Conjugation:
  • Endotoxin:
  • Purity:
  • Fc Engineering:
  • Published Data
  • Gene Expression
  • Datasheet
  • MSDS
  • COA
FC

Figure 1 Establishment of mouse anti-hCLDN1 MAbs.

Figure 1 Establishment of mouse anti-hCLDN1 MAbs.

Huh7.5.1-8 (black histograms) or S7-A (white histograms) cells were incubated with the conditioned medium from each hybridoma clone and treated with phycoerythrin-conjugated secondary Abs. Stained cells were analyzed by flow cytometry.

Fukasawa, M., Nagase, S., Shirasago, Y., Iida, M., Yamashita, M., Endo, K., ... & Kuniyasu, H. (2015). Monoclonal antibodies against extracellular domains of claudin-1 block hepatitis C virus infection in a mouse model. Journal of virology, 89(9), 4866-4879.

ELISA

Figure 2 Measurement of anti-CLDN1 MAb binding to cellular CLDN1 by cell ELISA.

Figure 2 Measurement of anti-CLDN1 MAb binding to cellular CLDN1 by cell ELISA.

After fixation, Huh7.5.1-8 cells in 96-well plates were incubated with serial dilutions of each anti-CLDN1 MAb (2C1, 3A2, 5F2, and 7A5) or control IgG, followed by horseradish peroxidase-conjugated second antibody.

Fukasawa, M., Nagase, S., Shirasago, Y., Iida, M., Yamashita, M., Endo, K., ... & Kuniyasu, H. (2015). Monoclonal antibodies against extracellular domains of claudin-1 block hepatitis C virus infection in a mouse model. Journal of virology, 89(9), 4866-4879.

IB

Figure 3 Cell lysates were subjected to immunoblotting of HCV NS3, core, and GAPDH proteins.

Figure 3 Cell lysates were subjected to immunoblotting of HCV NS3, core, and GAPDH proteins.

Huh7.5.1-8 cells were seeded at 5×10^4 cells in 48-well plates and pretreated with the indicated amount of anti-hCLDN1 MAb or control IgG (control Ab) for 1 h at room temperature and then were subjected to the following procedures. Cells were infected with HCVcc (MOI = 1) for 2 h at room temperature and cultured for 4 days in the presence of the indicated amounts (0.1 to 5 μg/well) of MAbs (400 μl/well).

Fukasawa, M., Nagase, S., Shirasago, Y., Iida, M., Yamashita, M., Endo, K., ... & Kuniyasu, H. (2015). Monoclonal antibodies against extracellular domains of claudin-1 block hepatitis C virus infection in a mouse model. Journal of virology, 89(9), 4866-4879.

Inhib

Figure 4 Inhibition of HCV infection by anti-hCLDN1 MAbs in vitro.

Figure 4 Inhibition of HCV infection by anti-hCLDN1 MAbs in vitro.

Cells were then infected with HCVpp (genotype 2a) for 3 h atroom temperature and cultured for 2 days in the presence of the indicated amounts of each anti-hCLDN1 MAb. Luciferase activities in cell lysates were measured using a luminometer. Values are expressed as percentages. Data in each graph represent the means ±SD (n=3). RLU, relative light units.

Fukasawa, M., Nagase, S., Shirasago, Y., Iida, M., Yamashita, M., Endo, K., ... & Kuniyasu, H. (2015). Monoclonal antibodies against extracellular domains of claudin-1 block hepatitis C virus infection in a mouse model. Journal of virology, 89(9), 4866-4879.

IF

Figure 5 Anti-CLDN1 MAbs did not affect TJ functions in cultured cells.

Figure 5 Anti-CLDN1 MAbs did not affect TJ functions in cultured cells.

Huh7.5.1-8 cells were treated with or without 5 μg of anti-hCLDN1 MAbs and cultured for 4 days at 37°C. Cells were fixed, permeabilized, and stained with anti-CLDN1 pAbs, which recognize the cytosolic domain of CLDN1.

Fukasawa, M., Nagase, S., Shirasago, Y., Iida, M., Yamashita, M., Endo, K., ... & Kuniyasu, H. (2015). Monoclonal antibodies against extracellular domains of claudin-1 block hepatitis C virus infection in a mouse model. Journal of virology, 89(9), 4866-4879.


Specifications

  • Immunogen
  • Eukaryotic expression vector that encoded hCLDN1
  • Host Species
  • Mouse
  • Specificity
  • Human
  • Clone
  • 5F2
  • Applications
  • FC, ELISA, IB, Inhib, IF
  • Related Disease
  • Hepatitis C

Product Property

  • Preservative
  • Human
  • Storage
  • CLDN1 (claudin-1)

Applications

  • Application Notes
  • This antibody has been reported for use in Flow Cytometry, Immonobloting, Inhibition, Immunofluorescence and Enzyme-Linked Immunosorbent Assay.

Target

  • Alternative Names
  • CLDN1; claudin 1; CLD1; SEMP1; ILVASC; claudin-1; senescence-associated epithelial membrane protein 1

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

Download resources about recombinant antibody development and antibody engineering to boost your research.

See other products for "Clone 5F2"

See other products for "CLDN1"

Single-domain Antibody

CAT Product Name Application Type
NAB-594-VHH Recombinant Anti-Human CLDN1 VHH Single Domain Antibody WB, IP, ICC, FC, FA Llama VHH

Recombinant Antibody

Rabbit Monoclonal Antibody

CAT Product Name Application Type
MOR-0716 Hi-Affi™ Rabbit Anti-CLDN1 Recombinant Antibody (clone DS716AB) IHC-P Rabbit IgG

Customer Reviews and Q&As

Submit a review or a question
There are currently no Customer reviews or questions for TAB-481LC. Click the button above to contact us or submit your feedback about this product.
View the frequently asked questions answered by Creative Biolabs Support.

For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

Send Inquiry

This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.

© 2024 Creative Biolabs.
  • 0
  • 0
Cart

    Go to compare