Recombinant Mouse Anti-EV71 VP0 Antibody (22A12)
CAT#: PABL-087
Recombinant Mouse Antibody (22A12) is capable of binding to EV71 VP0, expressed in HEK 293 cells.
Published Data
Figure 1 As the amount of MAb increases, infectivity decreases, and there are significant differences in the neutralization of inoculum and purified infectious virus.
EV71 infectious virus preincubated with MAb 22A12 (red line) was incapable of infecting HeLa cells at all but one tested concentration of antibody, indicating efficient neutralization of purified virus. However, the EV71 inoculum (a mixture of infectious virus and procapsid) preincubated with the same amount of MAb 22A12 (blue line) readily infected cells, causing severe CPE and death.
Shingler, K. L., Cifuente, J. O., Ashley, R. E., Makhov, A. M., Conway, J. F., & Hafenstein, S. (2015). The enterovirus 71 procapsid binds neutralizing antibodies and rescues virus infection in vitro. Journal of virology, 89(3), 1900-1908.
Figure 2 EV71 procapsid inhibits neutralization of infectious virus by MAb 22A12.
Purified EV71 infectious virus (1 105 mg/ml) preincubated with MAb 22A12 (6.25 102 mg/ml) was incapable of infecting HeLa cells. As increasing amounts of purified procapisd were added to the mixture of virus and antibody, the infectivity level of infectious virus increased, causing severe CPE and death of HeLa cells. The x axis indicates the number of procapsids present per infectious virus.
Shingler, K. L., Cifuente, J. O., Ashley, R. E., Makhov, A. M., Conway, J. F., & Hafenstein, S. (2015). The enterovirus 71 procapsid binds neutralizing antibodies and rescues virus infection in vitro. Journal of virology, 89(3), 1900-1908.
Figure 3 The antigenically different EV71 procapsid (yellow) binds efficiently and sequesters neutralizing MAb 22A12 (blue) in vitro. This preferential binding minimizes the amount of unbound MAb, which protects infectious virus (red), allowing successful binding to susceptible cells to initiate an infection. This result suggests a model for a potential function of procapsid to enhance infectious virus infectivity because of altered antigenicity between the two capsid forms.
Kristin L. Shingler,a Javier O. Cifuente,a Robert E. Ashley,a Alexander M. Makhov,b James F. Conway,b Susan Hafensteina
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Specifications
- Immunogen
- Enterovirus 71 Capsid protein VP0
- Host Species
- Mouse
- Derivation
- Mouse
- Type
- IgG
- Specificity
- Tested positive against native EV71 VP0
- Species Reactivity
- EV71
- Clone
- 22A12
- Applications
- Neut, FuncS
Product Property
- Purity
- >95% by SDS-PAGE and HPLC analysis
- Storage
- Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing of samples.
Applications
- Application Notes
- The antibody was validated for Neut. For details, refer to published data.
Target
- Alternative Names
- Capsid protein VP0; VP0
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PSBL-087 | Recombinant Human Anti-EV71 VP0 Antibody scFv Fragment (22A12) | Neut, FuncS | scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PFBL-087 | Recombinant Human Anti-EV71 VP0 Antibody Fab Fragment (22A12) | Neut, FuncS | Fab |
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