Recombinant Human Anti-GET3 Antibody (sAB) (CAT#: PABL-102)
Recombinant Human Antibody (sAB) is capable of binding to GET3, expressed in HEK 293 cells. Expressed as the combination of a heavy chain (HC) containing VH from anti-GET3 mAb and CH1-3 region of human IgG1 and a light chain (LC) encoding VL from anti-GET3 mAb and CL of human kappa light chain. Exists as a disulfide linked dimer of the HC and LC hetero-dimer under non-reducing condition.
Specific Inquiry
Figure 1 C) Single point competition ELISA - scatter plot showing ELISA signals of target binding (x-axis) and competition with soluble protein (y-axis) - clones located in top left corner of the plot (high ELISA 450 nm signal and low competition ratio; positive clone threshold - dashed line in gray) are picked for secondary screening (marked in blue).
Paduch, M., Koide, A., Uysal, S., Rizk, S. S., Koide, S., & Kossiakoff, A. A. (2013). Generating conformation-specific synthetic antibodies to trap proteins in selected functional states. Methods, 60(1), 3-14.
Figure 2 D) SPR - affinity measurement; plot represents SPR trace of sAB injection (3 analyte concentrations) over 4 channels on a chip - binding is detected only when target protein (Abh3) is crosslinked to its ligand (DNA) - immobilized on channel 2 (blue lines), sABs do not bind to the reference channel or ligand alone and only residual binding (small signal change) is observed for protein without the ligand (red lines).
Paduch, M., Koide, A., Uysal, S., Rizk, S. S., Koide, S., & Kossiakoff, A. A. (2013). Generating conformation-specific synthetic antibodies to trap proteins in selected functional states. Methods, 60(1), 3-14.
Figure 3 E) DSF - thermal shift assay: red line - target, blue line - target-sAB complex melt curves, increased stability of target protein is observed in the presence of the excess of sAB indicated by thermal shift (black arrow, 15 °C shift).
Paduch, M., Koide, A., Uysal, S., Rizk, S. S., Koide, S., & Kossiakoff, A. A. (2013). Generating conformation-specific synthetic antibodies to trap proteins in selected functional states. Methods, 60(1), 3-14.
Figure 4 F) size exclusion chromatography - complex formation is observed by large volumetric shift (black arrow) of target-sAB (blue line) complex on elution profile.
Paduch, M., Koide, A., Uysal, S., Rizk, S. S., Koide, S., & Kossiakoff, A. A. (2013). Generating conformation-specific synthetic antibodies to trap proteins in selected functional states. Methods, 60(1), 3-14.
Specifications
- Immunogen
- The details of the immunogen for this antibody are not available.
- Host Species
- Human
- Derivation
- Human
- Type
- IgG
- Specificity
- Tested positive against native S. cerevisiae GET3
- Species Reactivity
- S. cerevisiae
- Clone
- sAB
- Applications
- WB, ELISA, FuncS
Product Property
- Purity
- >95% by SDS-PAGE and HPLC analysis
- Storage
- Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing of samples.
Applications
- Application Notes
- The antibody was validated for ELISA. For details, refer to published data.
Target
- Alternative Names
- ARR4; ATPase GET3; GET3
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PSBL-102 | Recombinant Human Anti-GET3 Antibody scFv Fragment (sAB) | WB, ELISA, FuncS | scFv |
Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PFBL-102 | Recombinant Human Anti-GET3 Antibody Fab Fragment (sAB) | WB, ELISA, FuncS | Fab |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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