Mouse Anti-GM3 Recombinant Antibody (clone 14F7) (CAT#: PABC-051)

Figure 1 Immunohistochemical detection of NeuGc-GM3 ganglioside with the 14F7 antibody, in positive control cells, X63 murine myeloma cell line and retinoblastoma cells, WERI‐Rb1 and Y79.

(A–C) Positive control shows intense immunoreactivity to 14F7 selectively localized in the cell membrane. (D–F) WERI‐Rb1 cells are also positive to the 14F7, but the immunolabel is located in small rounded structures resembling lipid rafts. (G–I) Y79 cells are also positive to 14F7, showing the same distribution of immunolabel than WERI‐Rb1, but with less intensity than the other one. Nuclear staining: DAPI. Calibration bar: 20 μm.

Torbidoni, A. V., Scursoni, A., Camarero, S., Segatori, V., Gabri, M., Alonso, D., ... & de Dávila, M. T. G. (2015). Immunoreactivity of the 14F7 Mab raised against N‐Glycolyl GM 3 Ganglioside in retinoblastoma tumours. Acta ophthalmologica, 93(4), e294-e300.

Figure 2 Microphotographies showing the immunoreactivity to 14F7 antibody against NeuGc‐GM3 ganglioside in two sections of non‐invasive retinoblastomas, non‐tumoural retina and the negative isotype control in a section of retinoblastoma tumour.

(A) In this section of non‐invasive retinoblastoma, the tumour cells have replaced the retina (re). All the tumour cells are positive to 14F7 in this type of retinoblastoma tumour. The retinal pigment epithelium (rpe) conserves its integrity, and the choroids and the sclera are free of tumour cells. Neither the choroids (ch) nor the sclera (scl) are positive to 14F7. (B) Non‐invasive retinoblastoma where the retina has been almost completely supplanted by tumour, just remaining a little‐folded piece of it. The tumour cells are positive to NeuGc‐GM3, independently of their differentiation degree. (C) In the non‐tumoural retina, all the layers are not immunoreactive to 14F7, neither the choroids nor the pigment retinal epithelium. (D) Negative isotype control is completely absence of immunoreactivity to 14F7. Calibration bar: 50 μm.

Torbidoni, A. V., Scursoni, A., Camarero, S., Segatori, V., Gabri, M., Alonso, D., ... & de Dávila, M. T. G. (2015). Immunoreactivity of the 14F7 Mab raised against N‐Glycolyl GM 3 Ganglioside in retinoblastoma tumours. Acta ophthalmologica, 93(4), e294-e300.

Figure 1 Mouse Anti-GM3 Recombinant Antibody (PABC-051) in SDS-PAGE

SDS-PAGE analysis of PABC-051 in non-reduced (Lane 1) and reduced (Lane 2) conditions.

Figure 2 Mouse Anti-GM3 Recombinant Antibody (PABC-051) in HPLC

HPLC analysis of PABC-051 shows the purity is>95%.