Recombinant Mouse Anti-HBeAg Antibody (e6) (CAT#: PABL-112)
Recombinant Mouse Antibody (e6) is capable of binding to HBeAg, expressed in Chinese Hamster Ovary cells (CHO).
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We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
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Figure 1 Epitope mapping using surface plasmon resonance.
(A) Biacore sensograms indicating binding kinetics generated from immobilized Fab e13 (ligand) titrated with analyte mixtures containing a fixed amount of rHBeAg (1.5 µM) and a variable amount of Fab me6 (0 – 133 nM). (B) Immobilized Fab me6 (ligand) titrated with analyte mixtures containing either a fixed amount (0.4 µM) of rHBeAg and a variable amount of Fab e13, or a fixed amount (1.5 µM) rHBeAg and a variable amount of Fab me6. The steady-state maximum binding is plotted as a function of analyte Fab concentration (abscissa). For both panels A and B, the ordinate scales indicate SPR response in resonance units (RU).
Zhuang, X., Watts, N. R., Palmer, I. W., Kaufman, J. D., Dearborn, A. D., Trenbeath, J. L.,... & Wingfield, P. T. (2017). Chimeric Rabbit/Human Fab Antibodies Against the Hepatitis B e-antigen and Their Potential Applications in Assay, Characterization and Therapy. Journal of Biological Chemistry, jbc-M117.
Figure 2 Secondary antibody pairing.
A sandwich ELISA was used which incorporated Mab e6-coated plates for antigen capture, and Fabs e13, e21 or e38 for detection. Anti-human IgGHRP was used to generate the absorbance signal at 450 nm. The assay was performed over the range of 0 – 1 µg/ml rHBeAg. The experiment was performed three times, with similar results.
Zhuang, X., Watts, N. R., Palmer, I. W., Kaufman, J. D., Dearborn, A. D., Trenbeath, J. L.,... & Wingfield, P. T. (2017). Chimeric Rabbit/Human Fab Antibodies Against the Hepatitis B e-antigen and Their Potential Applications in Assay, Characterization and Therapy. Journal of Biological Chemistry, jbc-M117.
Specifications
- Immunogen
- Hepatitis B e-Antigen
- Host Species
- Mouse
- Derivation
- Mouse
- Type
- IgG
- Specificity
- Tested positive against native HBV HBeAg
- Species Reactivity
- HBV
- Clone
- e6
- Applications
- Can be useful in applications such as: Western blot; Enzyme-linked Immunosorbent Assay; Functional Study
Product Property
- Purity
- >95% by SDS-PAGE and HPLC analysis
- Storage
- Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing of samples.
Target
- Alternative Names
- Hepatitis B e-Antigen; HBeAg
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| IAB-B037(A) | Recombinant Anti-HBeAg Intrabody [(D-Arg)9] | WB, ICC, FuncS | vhh-(D-Arg)9 |
| IAB-B037(G) | Recombinant Anti-HBeAg Intrabody [+36 GFP] | ICC, Neut, FuncS | vhh-(+36GFP) |
| IAB-B037(T) | Recombinant Anti-HBeAg Intrabody [Tat] | ELISA, WB, ICC, FuncS | vhh-Tat |
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