Recombinant Human Anti-HCMV AD-2S1 epitope of gB Antibody (8F9) (CAT#: PABZ-056)
Recombinant Human Antibody (8F9) is capable of binding to HCMV AD-2S1 epitope of gB, expressed in HEK 293 cells. Expressed as the combination of a heavy chain (HC) containing VH from anti-HCMV AD-2S1 epitope of gB mAb and CH1-3 region of human IgG1 and a light chain (LC) encoding VL from anti-HCMV AD-2S1 epitope of gB mAb and CL of human kappa light chain. Exists as a disulfide linked dimer of the HC and LC hetero-dimer under non-reducing condition. The hypermutated, high-affinity, neutralizing human monoclonal antibody 8F9 againsts the AD-2S1 epitope of gB for which sequence information is known, uses two highly conserved V-genes.
Specific Inquiry
Figure 1 Dissociation of 8F9 Fab from cells expressing the N‐terminal amino acids (residues 28–100) of gB.
(A) Binding of the 8F9 Fab determined by flow cytometry, with comparisons to unstained cells and cells stained with a secondary anti‐human kappa antibody alone. (B) Binding of the 8F9 Fab to the N‐terminal fragment of gB at time zero and after 2 h of dissociation at 4, 14, 25 and 37°C.
Thomson, C. A., Bryson, S., McLean, G. R., Creagh, A. L., Pai, E. F., & Schrader, J. W. (2008). Germline V‐genes sculpt the binding site of a family of antibodies neutralizing human cytomegalovirus. The EMBO Journal, 27(19), 2592-2602.
Figure 2 ELISA comparing the binding of 8F9, M2J1 and M2J5 Fabs to plates coated with AD-2S1.
Thomson, C. A., Bryson, S., McLean, G. R., Creagh, A. L., Pai, E. F., & Schrader, J. W. (2008). Germline V‐genes sculpt the binding site of a family of antibodies neutralizing human cytomegalovirus. The EMBO Journal, 27(19), 2592-2602.
Specifications
- Immunogen
- HCMV Envelope glycoprotein B
- Host Species
- Human
- Derivation
- Human
- Type
- IgG
- Specificity
- Tested positive against native HCMV AD-2S1 epitope of gB
- Species Reactivity
- HCMV
- Clone
- 8F9
- Applications
- FC Assay-Dependent
ELISA Assay-Dependent
Neut Assay-Dependent
Product Property
- Purity
- >95% by SDS-PAGE and HPLC analysis
- Storage
- Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing of samples.
Applications
- Application Notes
- The antibody 8F9 has been reported in applications of FC, ELISA, Neut. It's recommended that the optimal antibody concentration, dilution, incubition time etc. are best to be carefully titrated in specific assays.
Target
- Alternative Names
- gB; Envelope glycoprotein B; Env; Envelope; glycoprotein B
- UniProt ID
- P06473
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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MHC Tetramer Products for Virology
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABL-114 | Recombinant Human Anti-HCMV glycoprotein B Antibody (1G2 ) | WB, ELISA, Neut, FuncS | IgG |
| PABL-115 | Recombinant Human Anti-HCMV Glycoprotein B Antibody (KE5) | WB, ELISA, IF, FuncS | IgG |
| PABL-116 | Recombinant Human Anti-HCMV glycoprotein B Antibody (SM5-1) | Neut, FuncS | IgG |
| FAMAB-0242-CN | Recombinant Chimeric (Mouse/Human) Anti-gB Antibody (CK1) | WB, ELISA | Chimeric (mouse/human) IgG1, Κ |
| FAMAB-0274-CN | Recombinant Mouse Anti-gB Antibody (gB-15) | ELISA | Mouse IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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