In the case of humoral immunity, epitopes, in particular antigenic domain-1, in glycoprotein B have proven to be important for the induction of virus-neutralizing activity. Such antibodies can exert potent virus-neutralizing activity but can also block neutralizing antibodies from binding. The clone, S2-6, showed recognition of most of the AD-1 mutant proteins comparable to wild type ITC52.
Figure 1 Reactivity of scFv S2-3 and S2-6 with prokaryotically expressed fusion proteins.
E. coli lysates were subjected to SDS-PAGE and analyzed in immunoblots, where the disulphide-linked epitope reforms under the prevailing oxidating conditions.
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