Recombinant Human Anti-HCMV glycoprotein B Antibody (1G2 )
CAT#: PABL-114
Recombinant Human Antibody (1G2 ) is capable of binding to HCMV glycoprotein B, expressed in HEK 293 cells. Expressed as the combination of a heavy chain (HC) containing VH from anti-HCMV glycoprotein B mAb and CH1-3 region of human IgG1 and a light chain (LC) encoding VL from anti-HCMV glycoprotein B mAb and CL of human kappa light chain. Exists as a disulfide linked dimer of the HC and LC hetero-dimer under non-reducing condition.
Published Data
Tested Data
Figure 2 FACS analysis of BHK cells electroporated with RNA encoding full-length gB and stained with monoclonal antibodies 1G2 and ITC88 24 h post electroporation. The gB staining histograms were gated on viable, dsRNA+ cells.
Chandramouli, S., Ciferri, C., Nikitin, P. A., Caló, S., Gerrein, R., Balabanis, K.,... & Carfi, A. (2015). Structure of HCMV glycoprotein B in the postfusion conformation bound to a neutralizing human antibody. Nature communications, 6, 8176.
Figure 3 Western blot analysis of purified VR1814 virions immunoprecipitated with 1G2 or ITC88. Samples were resolved on SDS–polyacrylamide gel electrophoresis and detected with ITC88 mAb. The left panel shows immunoprecipitation of soluble gB-698glyco by both antibodies as control.
Chandramouli, S., Ciferri, C., Nikitin, P. A., Caló, S., Gerrein, R., Balabanis, K.,... & Carfi, A. (2015). Structure of HCMV glycoprotein B in the postfusion conformation bound to a neutralizing human antibody. Nature communications, 6, 8176.
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SDS-PAGE
Figure 1 Recombinant Human Anti-HCMV glycoprotein B Antibody (PABL-114) in SDS-PAGE
SDS-PAGE analysis of PABL-114 in non-reduced (Lane 1) and reduced (Lane 2) conditions.
SEC-HPLC
Figure 2 Recombinant Human Anti-HCMV glycoprotein B Antibody (PABL-114) in HPLC
HPLC analysis of PABL-114 shows the purity is>95%.
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Specifications
- Immunogen
- HCytomegalovirus glycoprotein B
- Host Species
- Human
- Derivation
- Human
- Type
- IgG
- Specificity
- Tested positive against native HCMV gB
- Species Reactivity
- HCMV
- Clone
- 1G2
- Applications
- FC, IP, WB, ELISA, Neut, FuncS
Product Property
- Purity
- >95% by SDS-PAGE and HPLC analysis
- Storage
- Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing of samples.
Applications
- Application Notes
- The antibody was validated for FC and IP. For details, refer to published data.
Target
- Alternative Names
- gB; glycoprotein B
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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