Recombinant Mouse Anti-RSV gp F Antibody (101F)
CAT#: PABL-694
Recombinant Mouse Antibody (101F) is capable of binding to RSV gp F , expressed in Chinese Hamster Ovary cells (CHO).
Published Data
Figure 1 Neutralization with anti-F MAbs added before (A) or during (B) HRSV infection.directed against the F protein of HRSV.
(A) Long virus (8.2 × 105 PFU) was incubated in the absence or presence of 400 μg of the anti-F MAbs indicated on the figure. Virus-antibody mixtures were ultracentrifuged (125,812 × g for 2 h in a Beckman SW60 rotor), and the pellets were resuspended and used to infect HEp-2 cells. After 72 h of incubation, production of viral antigen was quantified by ELISA, as described in Materials and Methods, and results are presented as a percentage of the value for control cells infected in the absence of antibody. MAb 1B.C11 against a capsid protein of ASPV was used as a negative control (C−) in neutralization. (B) Long virus (1 × 105 PFU) ultracentrifuged as before in the absence of antibody was used to infect HEp-2 cells in the absence or presence of 3 μg of the antibodies indicated on the figure. Production of viral antigen was quantified as described for panel A. Data represent the mean and standard deviation from three independent experiments.
Magro, M. , Andreu, D. , Paulino GómezPuertas, José A Melero, & Concepción Palomo. (2010). Neutralization of human respiratory syncytial virus infectivity by antibodies and low-molecular-weight compounds targeted against the fusion glycoprotein. Journal of Virology, 84(16), 7970-7982.
Figure 2 Antigen binding and virus neutralization with MAbs directed against the F protein of HRSV.
Serial dilutions of purified anti-F MAbs were tested in a direct ELISA for binding to a soluble form of the F protein (FTM−), as described in Materials and Methods.
Magro, M. , Andreu, D. , Paulino GómezPuertas, José A Melero, & Concepción Palomo. (2010). Neutralization of human respiratory syncytial virus infectivity by antibodies and low-molecular-weight compounds targeted against the fusion glycoprotein. Journal of Virology, 84(16), 7970-7982.
Figure 3 Quantification of virus binding to cells and infectivity in the presence of MAbs.
(A) Long virus (6.5 × 105 PFU) preincubated in the absence or presence of 30 μg of the antibodies indicated on the figure, was mixed with a suspension of HEp-2 cells for 1 h at 4°C, and the virus bound to cells was detected by flow cytometry using an anti-F Cy5 antibody. (B) Aliquots of the virus-antibody-cell mixtures were replated. Forty-eight hours postinfection, cells were resuspended and labeled with an anti-F Cy5 antibody. Mock-infected cells or cells incubated with the MAb 63G against the attachment (G) glycoprotein or with the MAb 1B.C11 (C−) against a capsid protein of ASPV were used as controls in the experiment.
Magro, M. , Andreu, D. , Paulino GómezPuertas, José A Melero, & Concepción Palomo. (2010). Neutralization of human respiratory syncytial virus infectivity by antibodies and low-molecular-weight compounds targeted against the fusion glycoprotein. Journal of Virology, 84(16), 7970-7982.
Figure 4 Inhibition of syncytium formation by anti-F MAbs.
(A) BSR-T7/5 cells were transfected with 500 ng of pTM1 plasmid encoding the full-length F gene. The transfection mixture was removed 7 h later, and the MAbs (40 μg/ml) indicated below each panel were added to the culture. Formation of syncytium was evaluated after incubation for 48 h, as described in Materials and Methods. (B) HEp-2 cells were infected with the Long strain of HRSV (multiplicity of infection, 0.1 PFU/cell). The inoculum was removed at 90 min postinfection, and cells were maintained with medium. Five hours later, MAbs were added to the culture. Syncytium formation was examined as described for panel A. The results shown in this figure are representative of three independent experiments.
Magro, M. , Andreu, D. , Paulino GómezPuertas, José A Melero, & Concepción Palomo. (2010). Neutralization of human respiratory syncytial virus infectivity by antibodies and low-molecular-weight compounds targeted against the fusion glycoprotein. Journal of Virology, 84(16), 7970-7982.
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Specifications
- Immunogen
- HRSV F
- Host Species
- Mouse
- Derivation
- Mouse
- Type
- IgG
- Specificity
- Tested positive against native RSV gp F
- Species Reactivity
- HRSV F
- Clone
- 101F
- Applications
- ELISA, Neut
Product Property
- Purity
- >95% by SDS-PAGE and HPLC analysis
- Storage
- Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing of samples.
Applications
- Application Notes
- The F antibody has been reported in applications of Neutralization, ELISA, FC, IF.
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User-Friendly with Excellent Support
Using this antibody is straightforward, thanks to the detailed product datasheet and excellent customer support offered by Creative Biolabs. Whenever we've had questions, their team has been prompt and helpful, ensuring our experiments run smoothly.
Outstanding Neutralization Efficacy
The 101F Anti-RSV gp F antibody has proven to be an indispensable asset in our RSV research lab, consistently delivering high specificity and outstanding neutralization capabilities. Its performance in ELISA and neutralization assays is reliable and reproducible, facilitating our ongoing studies on RSV fusion inhibition.
Perfect for Cellular Studies
We've used this antibody primarily in immunofluorescence assays, and it has consistently revealed detailed insights into RSV F protein localization within infected cells. Its specificity has greatly enhanced the accuracy of our cell-based assays.
Q&As
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What are the benefits of using a recombinant antibody in this research?
A: Recombinant antibodies like this provide increased sensitivity, specificity, and consistency. Their animal-free production and enhanced batch consistency make them especially appealing for detailed and reproducible research.
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Why would researchers choose this IgG over other formats?
A: The IgG format of this antibody offers enhanced stability and ideal performance in a variety of immunochemical applications, including ELISA, flow cytometry, and immunofluorescence, providing researchers with versatile tool for detecting viral antigens.
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How is this antibody typically used in research?
A: This antibody is commonly used in neutralization assays and enzyme-linked immunosorbent assays (ELISA) to study the viral infectivity and assess the inhibition of RSV. Its specificity to the RSV F protein allows for precise characterization of viral processes in vitro.
View the frequently asked questions answered by Creative Biolabs Support.
Cite This Product
To accurately reference this product in your publication, please use the following citation information:
(Creative Biolabs Cat# PABL-694, RRID: AB_3111669)
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
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Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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See other products for "Clone 101F"
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| PFBL-687 | Recombinant Mouse Anti-RSV gp F Antibody Fab Fragment (101F) | ELISA, Neut | Fab |
| PSBC-209 | Recombinant Mouse Anti-RSV F Antibody scFv Fragment (101F) | Neut | scFv |
| HPAB-0139-YJ-F(E) | Mouse Anti-F Recombinant Antibody; Fab Fragment (HPAB-0139-YJ-F(E)) | Neut, Inhib, WB, IP | Mouse Fab |
| HPAB-0140-YJ-F(E) | Human Anti-F Recombinant Antibody; Fab Fragment (HPAB-0140-YJ-F(E)) | Neut, Inhib, WB, IP | Human Fab |
| HPAB-0141-YJ-F(E) | Human Anti-F Recombinant Antibody; Fab Fragment (HPAB-0141-YJ-F(E)) | Neut, Inhib, WB, IP | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PSBL-687 | Recombinant Mouse Anti-RSV gp F Antibody scFv Fragment (101F) | ELISA, Neut | scFv |
| PFBC-209 | Recombinant Mouse Anti-RSV F Antibody Fab Fragment (101F) | Neut | Fab |
| PFBC-215 | Recombinant Human Anti-RSV F Antibody Fab Fragment (B21m) | Neut | Fab |
| HPAB-0139-YJ-S(P) | Mouse Anti-F Recombinant Antibody; scFv Fragment (HPAB-0139-YJ-S(P)) | Neut, Inhib, WB, IP | Mouse scFv |
| HPAB-0140-YJ-S(P) | Human Anti-F Recombinant Antibody; scFv Fragment (HPAB-0140-YJ-S(P)) | Neut, Inhib, WB, IP | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABC-139 | Human Anti-F Recombinant Antibody (PABC-139) | Neut | Human IgG |
| HPAB-0139-YJ | Mouse Anti-F Recombinant Antibody (HPAB-0139-YJ) | Neut, Inhib, WB, IP | Mouse IgG1, κ |
| HPAB-0140-YJ | Human Anti-F Recombinant Antibody (HPAB-0140-YJ) | Neut, Inhib, WB, IP | Human IgG1, κ |
| HPAB-0141-YJ | Human Anti-F Recombinant Antibody (HPAB-0141-YJ) | Neut, Inhib, WB, IP | Human IgG1, κ |
| VS-0625-FY518 | Mouse Anti-F Recombinant Antibody (clone 2506W0413) | ELISA | Mouse IgG |
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