Recombinant Human Anti-ZEBOV GP Antibody (KZ52) (CAT#: PABW-149)
Recombinant Human Antibody (KZ52) is capable of binding to ZEBOV GP, expressed in HEK 293 cells. Expressed as the combination of a heavy chain (HC) containing VH from anti-ZEBOV GP mAb and CH1-3 region of human IgG and a light chain (LC) encoding VL from anti-ZEBOV GP proteins mAb and CL of human kappa light chain. Exists as a disulfide linked dimer of the HC and LC hetero-dimer under non-reducing condition. KZ52 is an antibody isolated from a human survivor of a 1995 outbreak in Kikwit, Democratic Republic of the Congo. This antibody neutralizes Zaire ebolavirus in vitro and offers protection from lethal EBOV challenge in rodent models, but has minimal effects on viral pathogenicity in non-human primates.
Specific Inquiry
IF
Figure 1 Stable expression of EBOV-GP on the surface of transduced MDCK-SIAT1 cells.
Immunofluorescence pictures of MDCK-SIAT1 (C and E) and E-SIAT (D and F) cells stained with KZ52 and an FITC-linked anti-human antibody.
Xiao, J. H., Rijal, P., Schimanski, L., Tharkeshwar, A. K., Wright, E., Annaert, W., & Townsend, A. (2018). Characterization of influenza virus pseudotyped with Ebolavirus glycoprotein. Journal of virology, 92(4), e00941-17.
Neut
Figure 2 Antibody neutralization of pseudotyped E-S-FLU virus, H5-S-FLU virus, and EBOV-GP-pseudotyped lentivirus.
Viruses were preincubated for 2 h with antibody KZ52 (anti-GP) or 21D85A (anti-H5) before MDCK-SIAT1 indicator cells were added. Antibodies were titrated in 2-fold dilutions from 10 μg/ml. After 24 h, infection was quantified by measuring eGFP expression (influenza virus) or luminescence (lentivirus). Titration curves are shown in panels A to C.
Xiao, J. H., Rijal, P., Schimanski, L., Tharkeshwar, A. K., Wright, E., Annaert, W., & Townsend, A. (2018). Characterization of influenza virus pseudotyped with Ebolavirus glycoprotein. Journal of virology, 92(4), e00941-17.
Specifications
- Immunogen
- ZBOV Spike glycoprotein
- Host Species
- Human
- Derivation
- Human
- Type
- IgG
- Specificity
- Tested positive against native ZEBOV GP protein
- Species Reactivity
- ZEBOV
- Clone
- KZ52
- Applications
- WB, ELISA, IF, Neut
Product Property
- Purity
- >95% by SDS-PAGE and HPLC analysis
- Storage
- Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing of samples.
Applications
- Application Notes
- The GP antibody has been reported in applications of Western Blot, Enzyme-linked Immunosorbent Assay, Immunofluorescence, Neutralization.
Target
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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MHC Tetramer Products for Virology
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-410 | Recombinant Anti-GP Antibody | ELISA, WB, IF, FuncS | IgG |
| PABC-134 | Recombinant Mouse Anti-Z. ebolavirus GP Antibody (13F6-1-2) | ELISA, Neut | IgG |
| VS-0423-XY11 | Human Anti-GP Recombinant Antibody (clone 2.1.1D7) | ELISA | Human IgG |
| VS-0423-XY12 | Human Anti-GP Recombinant Antibody (clone 2.1.7G7) | ELISA | Human IgG |
| VS-0423-XY13 | Human Anti-GP Recombinant Antibody (clone 5.1.9E3) | ELISA | Human IgG |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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