What is the primary application of the Chicken Anti-PPAR gamma Antibody?

The primary application of this antibody is in molecular biology research. It is used for Western Blot (WB) techniques to study protein expression and function in various tissue types. Its main advantage is its high specificity for the PPAR gamma protein in human tissues.

What are the purification and quality standards of this antibody?

The antibody is purified using size-exclusion chromatography with a purity of over 95%, ensuring that it meets high-quality benchmarks. This level of purity is essential for ensuring experimental reliability and repeatability in research settings.

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PPARA
Chicken Anti-PPAR gamma Polyclonal IgY

Chicken Anti-PPAR gamma Polyclonal IgY (CAT#: BRD-0845MZ)

This antibody is a chicken polyclonal antibody which specifically reacts with PPAR gamma.

Gene Expression
Datasheet
MSDS
COA

Subcellular Location and Protein Expression

Figure 1 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/58901/if_selected.jpg

Figure 2 IHC staining of human cerebral cortex
(Immunohistochemical staining of human cerebral cortex shows nuclear positivity in neuronal cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/75701/160107_B_9_5_selected.jpg

Normal Tissue

Figure 3 Cerebral cortex
(Endothelial cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Glial cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Nuclear
Neuronal cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/75701/160107_B_9_5.jpg

Figure 4 Colon
(Endothelial cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Peripheral nerve/ganglion
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/75701/160107_A_7_3.jpg

Figure 5 Liver
(Cholangiocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Hepatocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/75701/160107_A_8_4.jpg

Figure 6 Kidney
(Cells in glomeruli
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Cells in tubules
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/75701/160107_A_8_5.jpg

Figure 7 Testis
(Preleptotene spermatocytes
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Round or early spermatids
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Sertoli cells
Staining: Low
Intensity: Moderate
Quantity: <25%
Spermatogonia cells
Staining: High
Intensity: Strong
Quantity:>75%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/75701/160107_A_6_6.jpg

Figure 8 Lymph node
(Germinal center cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Non-germinal center cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/75701/160107_A_8_8.jpg

RNA Expression

Figure 9 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000186951-PPARA/subcellular

Datasheet

Certificate of Analysis Lookup
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number

Specifications

Immunogen
Synthetic peptide based on human PPARg protein

Host Species
Chicken

Type
Chicken antibody

Species Reactivity
Human

Applications
WB

Target

Alternative Names
PPAR; NR1C1; hPPAR; PPARalpha

Gene ID
5465

UniProt ID
Q07869

Related Resources

Related Signaling Pathways

PPAR Signaling Pathway

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

Download resources about recombinant antibody development and antibody engineering to boost your research.

See other products for "PPARA"

Select a product category from the dropdown menu below to view related products.

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IgG Antibody Products

CAT	Product Name	Application	Type
MOB-3743z	Mouse Anti-PPARA Recombinant Antibody (clone 42H1)	WB, ICC, IF, FC, IP, FuncS	Mouse IgG2b
MOB-1489CT	Recombinant Mouse anti-Human PPARA Monoclonal antibody (I0834)	ChIP, ELISA, EMSA, IP, WB
VS3-QX896	Mouse Anti-PPARA Recombinant Antibody (clone 1331CT894.186.143)	WB, ICC, IHC, FC	Mouse IgG1, κ
VS3-WK895	Mouse Anti-PPARA Recombinant Antibody (VS3-WK895)	WB, FC, IF, IHC-P	Mouse IgG1, κ

Customer Reviews and Q&As

Customer Review Q&As

High Quality and Reliability

I have used this antibody in our lab's Western Blots consistently. The purity and high specificity improve our protein detection dramatically, minimizing background noise and increasing our data's credibility. It's a top-tier product for any project requiring antibody-based detection.

Great for Molecular Studies

This antibody has played a significant role in advancing our understanding of cancer metabolism. Its precision in binding PPAR gamma allows us to explore new pathways and regulatory processes with much greater clarity, providing a solid groundwork for developing targeted therapies.

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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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