Chicken Anti-PPAR gamma Polyclonal IgY
CAT#: BRD-0845MZ
This antibody is a chicken polyclonal antibody which specifically reacts with PPAR gamma.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/58901/if_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IHC staining of human cerebral cortex
Immunohistochemical staining of human cerebral cortex shows nuclear positivity in neuronal cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/75701/160107_B_9_5_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Endothelial cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Glial cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Nuclear
Neuronal cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/75701/160107_B_9_5.jpg
Normal Tissue
Figure 4 Colon
Endothelial cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Peripheral nerve/ganglion
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/75701/160107_A_7_3.jpg
Normal Tissue
Figure 5 Liver
Cholangiocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Hepatocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/75701/160107_A_8_4.jpg
Normal Tissue
Figure 6 Kidney
Cells in glomeruli
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Cells in tubules
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/75701/160107_A_8_5.jpg
Normal Tissue
Figure 7 Testis
Preleptotene spermatocytes
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Round or early spermatids
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Sertoli cells
Staining: Low
Intensity: Moderate
Quantity: <25%
Spermatogonia cells
Staining: High
Intensity: Strong
Quantity:>75%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/75701/160107_A_6_6.jpg
Normal Tissue
Figure 8 Lymph node
Germinal center cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Non-germinal center cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/75701/160107_A_8_8.jpg
RNA Expression
Figure 9 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000186951-PPARA/subcellular
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Specifications
- Immunogen
- Synthetic peptide based on human PPARg protein
- Host Species
- Chicken
- Type
- Chicken antibody
- Species Reactivity
- Human
- Applications
- WB
Target
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High Quality and Reliability
I have used this antibody in our lab's Western Blots consistently. The purity and high specificity improve our protein detection dramatically, minimizing background noise and increasing our data's credibility. It's a top-tier product for any project requiring antibody-based detection.
Great for Molecular Studies
This antibody has played a significant role in advancing our understanding of cancer metabolism. Its precision in binding PPAR gamma allows us to explore new pathways and regulatory processes with much greater clarity, providing a solid groundwork for developing targeted therapies.
Q&As
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What is the primary application of the Chicken Anti-PPAR gamma Antibody?
A: The primary application of this antibody is in molecular biology research. It is used for Western Blot (WB) techniques to study protein expression and function in various tissue types. Its main advantage is its high specificity for the PPAR gamma protein in human tissues.
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What are the purification and quality standards of this antibody?
A: The antibody is purified using size-exclusion chromatography with a purity of over 95%, ensuring that it meets high-quality benchmarks. This level of purity is essential for ensuring experimental reliability and repeatability in research settings.
View the frequently asked questions answered by Creative Biolabs Support.
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Related Signaling Pathways
PPAR Signaling Pathway
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
See other products for "PPARA"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-3743z | Mouse Anti-PPARA Recombinant Antibody (clone 42H1) | WB, ICC, IF, FC, IP, FuncS | Mouse IgG2b |
| MOB-1489CT | Recombinant Mouse anti-Human PPARA Monoclonal antibody (I0834) | ChIP, ELISA, EMSA, IP, WB | |
| VS3-QX896 | Mouse Anti-PPARA Recombinant Antibody (clone 1331CT894.186.143) | WB, ICC, IHC, FC | Mouse IgG1, κ |
| VS3-WK895 | Mouse Anti-PPARA Recombinant Antibody (VS3-WK895) | WB, FC, IF, IHC-P | Mouse IgG1, κ |
| VS7-0425-WR654 | Mouse Anti-PPARA Recombinant Antibody (clone 5D10E10) | FC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY5590 | Anti-PPARA Immunohistochemistry Kit | IHC |
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