Mouse Anti-PPARA Recombinant Antibody (clone 5D10E10)
CAT#: VS7-0425-WR654
This product is a mouse IgG1 antibody that binds to human PPARA. The antibody 5D10E10 recognizes specific amino acid domain of human PPARA (AA: 1-120). It can be used for FC assay.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/58901/if_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IHC staining of human cerebral cortex
Immunohistochemical staining of human cerebral cortex shows nuclear positivity in neuronal cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/75701/160107_B_9_5_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Endothelial cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Glial cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Nuclear
Neuronal cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/75701/160107_B_9_5.jpg
Normal Tissue
Figure 4 Colon
Endothelial cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Peripheral nerve/ganglion
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/75701/160107_A_7_3.jpg
Normal Tissue
Figure 5 Liver
Cholangiocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Hepatocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/75701/160107_A_8_4.jpg
Normal Tissue
Figure 6 Kidney
Cells in glomeruli
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Cells in tubules
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/75701/160107_A_8_5.jpg
Normal Tissue
Figure 7 Testis
Preleptotene spermatocytes
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Round or early spermatids
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Sertoli cells
Staining: Low
Intensity: Moderate
Quantity: <25%
Spermatogonia cells
Staining: High
Intensity: Strong
Quantity:>75%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/75701/160107_A_6_6.jpg
Normal Tissue
Figure 8 Lymph node
Germinal center cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Non-germinal center cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/75701/160107_A_8_8.jpg
RNA Expression
Figure 9 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000186951-PPARA/subcellular
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Specifications
- Immunogen
- Recombinant human PPARA (AA: 1-120) fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human PPARA
- Species Reactivity
- Human
- Clone
- 5D10E10
- Molecule Class
- Peroxisome Proliferator-Activated Receptor (Ppar) Nuclear Receptor
- Applications
- FC
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- 0.05% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:500 - 1:2000
Flow Cytometry: 1:200 - 1:400
Enzyme-linked Immunosorbent Assay: 1:10000
Target
- Alternative Names
- PPARA; Peroxisome Proliferator-Activated Receptor Alpha; PPAR; PPAR-Alpha; HPPAR; Peroxisome Proliferative Activated Receptor, Alpha; Peroxisome Proliferator-Activated Nuclear Receptor Alpha Variant 3
- Gene ID
- 5465
- UniProt ID
- Q07869
- Long Name
- Peroxisome proliferator activated receptor alpha
- Cellular Localization
- Nucleus
- Post Translation Modifications
- Ubl conjugation
- Protein Refseq
- NP_001001928.1
- Function
- Activator; DNA-binding; Receptor
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Related Signaling Pathways
PPAR Signaling Pathway
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
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- Product Name
Secondary Antibody
- CAT
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Reconstitution Buffer
- CAT
- Product Name
Recommended Dilution Buffer
- CAT
- Product Name
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|---|---|---|---|
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| MOB-1489CT | Recombinant Mouse anti-Human PPARA Monoclonal antibody (I0834) | ChIP, ELISA, EMSA, IP, WB | |
| VS3-QX896 | Mouse Anti-PPARA Recombinant Antibody (clone 1331CT894.186.143) | WB, ICC, IHC, FC | Mouse IgG1, κ |
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| VS7-0425-WR655 | Mouse Anti-PPARA Recombinant Antibody (clone 5D10C3) | WB, FC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0845MZ | Chicken Anti-PPAR gamma Polyclonal IgY | WB | Chicken antibody |
| CAT | Product Name | Application | Type |
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