Rabbit Anti-Actin Beta Recombinant Antibody (VS3-CJ1)
CAT#: VS3-CJ1
This product is a rabbit antibody that recognizes 43KDa beta-actin from Human.
Specifications
- Immunogen
- Recombinant beta-actin protein from bacteria
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human Actin Beta
- Species Reactivity
- Human
- Applications
- WB
- Conjugate
- Unconjugated
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Concentration
- 1 mg/mL (lot specific)
- Buffer
- 50% Glycerol, 150mM NaCl, PBS, pH7.4.
- Preservative
- 0.02% sodium azide
- Storage
- Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- This antibody has been tested for use in Western Blot (1:1000-1:4000).
Target
- Alternative Names
- BRWS1; PS1TP5BP1; ACTB
- Gene ID
- 60
- UniProt ID
- P60709
- Sequence Similarities
- Belongs to the actin family.
- Cellular Localization
- Cytoplasm, Cytoskeleton, Nucleus
- Post Translation Modifications
- ISGylated.
Oxidation of Met-44 and Met-47 by MICALs (MICAL1, MICAL2 or MICAL3) to form methionine sulfoxide promotes actin filament depolymerization. MICAL1 and MICAL2 produce the (R)-S-oxide form. The (R)-S-oxide form is reverted by MSRB1 and MSRB2, which promote actin repolymerization.
Monomethylation at Lys-84 (K84me1) regulates actin-myosin interaction and actomyosin-dependent processes (PubMed:23673617).
Demethylation by ALKBH4 is required for maintaining actomyosin dynamics supporting normal cleavage furrow ingression during cytokinesis and cell migration (PubMed:23673617).
Methylated at His-73 by SETD3 (PubMed:30526847, PubMed:30626964, PubMed:30785395, PubMed:31388018).Methylation at His-73 is required for smooth muscle contraction of the laboring uterus during delivery (By similarity).
Actin, cytoplasmic 1, N-terminally processed
N-terminal acetylation by NAA80 affects actin filament depolymerization and elongation, including elongation driven by formins (PubMed:29581253).
In contrast, filament nucleation by the Arp2/3 complex is not affected (PubMed:29581253).
(Microbial infection) Monomeric actin is cross-linked by V.cholerae toxins RtxA and VgrG1 in case of infection: bacterial toxins mediate the cross-link between Lys-50 of one monomer and Glu-270 of another actin monomer, resulting in formation of highly toxic actin oligomers that cause cell rounding (PubMed:19015515).
The toxin can be highly efficient at very low concentrations by acting on formin homology family proteins: toxic actin oligomers bind with high affinity to formins and adversely affect both nucleation and elongation abilities of formins, causing their potent inhibition in both profilin-dependent and independent manners (PubMed:26228148).
- Protein Refseq
- NP_001092.1
- Function
- Actin is a highly conserved protein that polymerizes to produce filaments that form cross-linked networks in the cytoplasm of cells (PubMed:29581253).
Actin exists in both monomeric (G-actin) and polymeric (F-actin) forms, both forms playing key functions, such as cell motility and contraction (PubMed:29581253).
In addition to their role in the cytoplasmic cytoskeleton, G- and F-actin also localize in the nucleus, and regulate gene transcription and motility and repair of damaged DNA (PubMed:29925947).
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
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- Product Name
Secondary Antibody
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See other products for "Actin Beta"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS3-CJ1063 | Mouse Anti-Actin Beta Recombinant Antibody (VS3-CJ1063), HRP | WB | Mouse IgG1 |
| VS3-CJ1064 | Rabbit Anti-Actin Beta Recombinant Antibody (VS3-CJ1064) | WB, ICC, IF, IHC | Rabbit IgG |
| VS3-CJ1065 | Mouse Anti-Actin Beta Recombinant Antibody (VS3-CJ1065) | WB, ICC, IF, IHC | Mouse IgG |
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