Rabbit Anti-ATXN3 Recombinant Antibody (clone R04-1I8)
CAT#: VS3-FY1972
We conducted western blot analysis of Ataxin 3 in Hela, A549, HL-60, U251, U87-MG lysates. It is a amazing rabbit recombinant monoclonal antibody that is engineered to detect human ATXN3. This R04-1I8 antibody is validated for western blot analysis of ataxin 3 in Hela, A549, HL-60, U251, U87-MG lysates.
Gene Expression
Tested Data
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & nucleoli.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/69338/1359_A7_2_selected.jpg
Normal Tissue
Figure 2 IHC staining of human rectum
Immunohistochemical staining of human rectum shows strong cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/24123/ihc_selected.jpg
Normal Tissue
Figure 3 IHC staining of human breast
Immunohistochemical staining of human breast shows strong positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/21976/ihc_selected.jpg
Normal Tissue
Figure 4 Cerebral cortex
Endothelial cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous nuclear Glial cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous nuclear Neuronal cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous nuclear Neuropil Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/21976/48177_B_7_5.jpg
Normal Tissue
Figure 5 Colon
Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear Peripheral nerve/ganglion Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/21976/48177_A_8_3.jpg
Normal Tissue
Figure 6 Kidney
Cells in glomeruli Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear Cells in tubules Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/21976/48177_A_8_5.jpg
Normal Tissue
Figure 7 Testis
Cells in seminiferous ducts Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear Leydig cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/21976/48177_A_5_6.jpg
Normal Tissue
Figure 8 Lymph node
Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/21976/48177_A_8_8.jpg
RNA Expression
Figure 9 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000066427-ATXN3
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WB
Figure 1 Anti-ATXN3 Antibody (VS3-FY1972) in WB.
The Ataxin 3 expression in Hela, A549, HL-60, U251, U87-MG lysates were detected using the anti-Ataxin 3 by western blot assay.
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Specifications
- Immunogen
- Synthetic peptide of human Ataxin 3
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human ATXN3
- Species Reactivity
- Human
- Clone
- R04-1I8
- Applications
- Western Blot
- MW
- The calculated weight of the target protein is approximately 41 kDa, while the observed molecular weight stands at 41 kDa.
- Related Disease
- Machado-Joseph disease
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Target
- Alternative Names
- Ataxin 3; Spinocerebellar Ataxia Type 3 Protein; Machado-Joseph Disease Protein 1; SCA3; ATX3; MJD1; MJD; Machado-Joseph Disease (Spinocerebellar Ataxia 3, Olivopontocerebellar Ataxia 3, Autosomal Dominant, Ataxin 3)
- Gene ID
- 4287
- UniProt ID
- P54252
- Cellular Localization
- Lysosome, Membrane, Nucleus
- Post Translation Modifications
- Monoubiquitinated N-terminally by UBE2W, possibly leading to activate the deubiquitinating enzyme activity (PubMed:23696636). (P54252-ATX3_HUMAN)
Ubiquitination at Lys190, Lys200, Lys117, and Lys291 (NX_P54252 [NX_P54252-2])
Modification sites at PhosphoSitePlus (P54252)
- Protein Refseq
- NP_001121168.1; NP_001121169.2; NP_001158246.1; NP_001158248.1; NP_001158249.1
- Function
- Binds long polyubiquitin chains and trims them, while it has weak or no activity against chains of 4 or less ubiquitins.
Involved in degradation of misfolded chaperone substrates via its interaction with STUB1/CHIP: recruited to monoubiquitinated STUB1/CHIP, and restricts the length of ubiquitin chain attached to STUB1/CHIP substrates and preventing further chain extension (By similarity).
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "ATXN3"
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| MOB-0695MZ | Mouse Anti-ATXN3 Recombinant Antibody (clone 0E2090.2) | ELISA | Mouse IgG1 |
| VS13-YC80 | Rabbit Anti-ATXN3 Recombinant Antibody (VS13-YC80) | IHC-P | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC1037 | AbPlus™ Anti-ATXN3 Magnetic Beads (VS-0724-YC1037) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY655 | Anti-ATXN3 Immunohistochemistry Kit | IHC |
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