Rabbit Anti-CDK5RAP3 Recombinant Antibody (clone R07-1A1)
CAT#: VS3-FY1637
This is a rabbit recombinant monoclonal antibody, which detects human CDK5RAP3. It cross-reacts with mouse. The R07-1A1 is recommended for dilution with western blot (dilution 1/500-1/1000), immunohistochemistry (dilution 1/50-1/100), immunoprecipitation (dilution 1/20).
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoli & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/22141/188_H9_2_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/27883/327_G4_1_selected.jpg
Normal Tissue
Figure 3 Testis
Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Pachytene spermatocytes Staining: High Intensity: Strong Quantity: 75%-25% Sertoli cells Staining: Medium Intensity: Moderate Quantity:>75%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/22141/50934_A_4_6.jpg
Normal Tissue
Figure 4 Epididymis
Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/22141/50934_A_2_6.jpg
Normal Tissue
Figure 5 Lymph node
Germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/22141/50934_A_9_8.jpg
Normal Tissue
Figure 6 Kidney
Cells in glomeruli Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/22141/50934_A_8_5.jpg
RNA Expression
Figure 7 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000108465-CDK5RAP3
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Specifications
- Immunogen
- Synthetic peptide of human CDK5RAP3
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human and Mouse CDK5RAP3
- Species Reactivity
- Human, Mouse
- Clone
- R07-1A1
- Applications
- Western Blot, Immunohistochemistry-Paraffin, Immunoprecipitation
- MW
- The calculated weight of the target protein is approximately 57 kDa, while the observed molecular weight stands at 70 kDa.
- Related Disease
- Beukes Hip Dysplasia and Renal Cysts And Diabetes Syndrome
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/50-1/100
Immunoprecipitation: 1/20
Target
- Alternative Names
- CDK5 Regulatory Subunit Associated Protein 3; LXXLL/Leucine-Zipper-Containing ARF-Binding Protein; LXXLL/Leucine-Zipper-Containing ARFbinding Protein; Ischemic Heart CDK5 Activator-Binding Protein C53; IC53; LZAP; CDK5 Regulatory Subunit Associated Protein IC53-2; CDK5 Regulatory Subunit-Associated Protein 3
- Gene ID
- 80279
- UniProt ID
- Q96JB5
- Sequence Similarities
- Belongs to the CDK5RAP3 family.
- Cellular Localization
- Cytoplasm, Cytoskeleton, Nucleus
- Post Translation Modifications
- Isopeptide bond, Phosphoprotein, Ubl conjugation
- Protein Refseq
- NP_001265126.1; NP_001265127.1; NP_001265145.1; NP_001265146.1; NP_788276.1
- Function
- Substrate adapter for ufmylation, the covalent attachment of the ubiquitin-like modifier UFM1 to substrate proteins, in response to endoplasmic reticulum stress.
Negatively regulates NF-kappa-B-mediated gene transcription through the control of RELA phosphorylation.
Probable tumor suppressor initially identified as a CDK5R1 interactor controlling cell proliferation.
Also regulates mitotic G2/M transition checkpoint and mitotic G2 DNA damage checkpoint.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "CDK5RAP3"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2476MZ | Recombinant Mouse Anti-Human CDK5RAP3 Antibody | IHC-P, WB | Mouse antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0633 | Hi-Affi™ Rabbit Anti-CDK5RAP3 Recombinant Antibody (clone DS633AB) | WB, IP, IHC-P, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY407 | Anti-CDK5RAP3 Immunohistochemistry Kit | IHC | |
| VS-0525-XY1320 | Anti-Human CDK5RAP3 Immunohistochemistry Kit | IHC |
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