Rabbit Anti-CDK5RAP3 Recombinant Antibody (clone R07-1A1) (CAT#: VS3-FY1637)
This is a rabbit recombinant monoclonal antibody, which detects human CDK5RAP3. It cross-reacts with mouse. The R07-1A1 is recommended for dilution with western blot (dilution 1/500-1/1000), immunohistochemistry (dilution 1/50-1/100), immunoprecipitation (dilution 1/20).
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Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoli & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/22141/188_H9_2_selected.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoli & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/22141/188_H9_2_selected.jpg
Figure 2 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/27883/327_G4_1_selected.jpg
(Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/27883/327_G4_1_selected.jpg
Figure 3 Testis
(Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Pachytene spermatocytes Staining: High Intensity: Strong Quantity: 75%-25% Sertoli cells Staining: Medium Intensity: Moderate Quantity:>75%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/22141/50934_A_4_6.jpg
(Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Pachytene spermatocytes Staining: High Intensity: Strong Quantity: 75%-25% Sertoli cells Staining: Medium Intensity: Moderate Quantity:>75%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/22141/50934_A_4_6.jpg
Figure 4 Epididymis
(Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/22141/50934_A_2_6.jpg
(Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/22141/50934_A_2_6.jpg
Figure 5 Lymph node
(Germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/22141/50934_A_9_8.jpg
(Germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/22141/50934_A_9_8.jpg
Figure 6 Kidney
(Cells in glomeruli Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/22141/50934_A_8_5.jpg
(Cells in glomeruli Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/22141/50934_A_8_5.jpg
Figure 7 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000108465-CDK5RAP3
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000108465-CDK5RAP3
Specifications
- Immunogen
- Synthetic peptide of human CDK5RAP3
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human and Mouse CDK5RAP3
- Species Reactivity
- Human, Mouse
- Clone
- R07-1A1
- Applications
- Western Blot, Immunohistochemistry-Paraffin, Immunoprecipitation
- MW
- The calculated weight of the target protein is approximately 57 kDa, while the observed molecular weight stands at 70 kDa.
- Related Disease
- Beukes Hip Dysplasia and Renal Cysts And Diabetes Syndrome
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/50-1/100
Immunoprecipitation: 1/20
Target
- Alternative Names
- CDK5 Regulatory Subunit Associated Protein 3; LXXLL/Leucine-Zipper-Containing ARF-Binding Protein; LXXLL/Leucine-Zipper-Containing ARFbinding Protein; Ischemic Heart CDK5 Activator-Binding Protein C53; IC53; LZAP; CDK5 Regulatory Subunit Associated Protein IC53-2; CDK5 Regulatory Subunit-Associated Protein 3
- Gene ID
- 80279
- UniProt ID
- Q96JB5
- Sequence Similarities
- Belongs to the CDK5RAP3 family.
- Cellular Localization
- Cytoplasm, Cytoskeleton, Nucleus
- Post Translation Modifications
- Isopeptide bond, Phosphoprotein, Ubl conjugation
- Protein Refseq
- NP_001265126.1; NP_001265127.1; NP_001265145.1; NP_001265146.1; NP_788276.1
- Function
- Substrate adapter for ufmylation, the covalent attachment of the ubiquitin-like modifier UFM1 to substrate proteins, in response to endoplasmic reticulum stress.
Negatively regulates NF-kappa-B-mediated gene transcription through the control of RELA phosphorylation.
Probable tumor suppressor initially identified as a CDK5R1 interactor controlling cell proliferation.
Also regulates mitotic G2/M transition checkpoint and mitotic G2 DNA damage checkpoint.
Recommended Products
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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IgG Antibody Products
Rabbit Monoclonal Antibody Products
IHC Kit and Antibody Products: Precision Tools for Immunohistochemistry
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2476MZ | Recombinant Mouse Anti-Human CDK5RAP3 Antibody | IHC-P, WB | Mouse antibody |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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