Rabbit Anti-ELOVL5 Recombinant Antibody (clone R07-7B3)

CAT#: VS3-FY1595

This anti-ELOVL5 antibody tested in western blot analysis of ELOVL5 in Jurkat, Hela lysates. A remarkable rabbit recombinant monoclonal antibody that detects human ELOVL5. The antibody is recommended for detection of ELOVL5 protein by WB, ICC/IF.

Tested Data
Figure 1 Anti-ELOVL5 Antibody (VS3-FY1595) in WB.

Specifications

  • Immunogen
  • Synthetic peptide of human ELOVL5
  • Host Species
  • Rabbit
  • Type
  • Rabbit IgG
  • Specificity
  • Human ELOVL5
  • Species Reactivity
  • Human
  • Clone
  • R07-7B3
  • Applications
  • Western Blot, Immunofluorescence
  • MW
  • The calculated weight of the target protein is approximately 35 kDa, while the observed molecular weight stands at 35 kDa.
  • Related Disease
  • Spinocerebellar Ataxia 38 and Intermittent Squint

Product Property

  • Purification
  • Affinity Purified
  • Format
  • Liquid
  • Concentration
  • Please refer to the vial label for the specific concentration.
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

  • Application Notes
  • Western Blot: 1/500-1/1000
    Immunofluorescence: 1/50-1/200

Target

  • Alternative Names
  • ELOVL Fatty Acid Elongase 5; ELOVL Family Member 5, Elongation Of Long Chain Fatty Acids (FEN1/Elo2, SUR4/Elo3-Like, Yeast); Very Long Chain 3-Ketoacyl-CoA Synthase 5; Very Long Chain 3-Oxoacyl-CoA Synthase 5; 3-Keto Acyl-CoA Synthase ELOVL5; Spinocerebellar Ataxia 38; Fatty Acid Elongase 1; ELOVL FA Elongase 5
  • Sequence Similarities
  • Belongs to the ELO family. ELOVL5 subfamily.
  • Cellular Localization
  • Cell projection, Endoplasmic reticulum, Membrane
  • Function
  • Catalyzes the first and rate-limiting reaction of the four reactions that constitute the long-chain fatty acids elongation cycle. This endoplasmic reticulum-bound enzymatic process allows the addition of 2 carbons to the chain of long- and very long-chain fatty acids (VLCFAs) per cycle. Condensing enzyme that acts specifically toward polyunsaturated acyl-CoA with the higher activity toward C18:3(n-6) acyl-CoA. May participate to the production of monounsaturated and of polyunsaturated VLCFAs of different chain lengths that are involved in multiple biological processes as precursors of membrane lipids and lipid mediators.
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Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

MSDS

COA

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Protocol & Troubleshooting

We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.

Isotype Control

Secondary Antibody

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