Rabbit Anti-ELP3 Recombinant Antibody (clone R03-1H8) (CAT#: VS3-XY3344)

• Host Species
• Rabbit

• Type
• Rabbit IgG

• Specificity
• Human, Mouse and Rat ELP3

• Species Reactivity
• Human, Mouse, Rat

• Clone
• R03-1H8

• Applications
• WB, ICC, IF, IP

• Related Disease
• Dysautonomia

• Clonality
• Monoclonal

• Aggregation
• < 5%

• Format
• Liquid

• Buffer
• PBS (pH 7.4), 150 mM NaCl and 50% glycerol

• Preservative
• 0.02% sodium azide

• Storage
• Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

• Shipping
• Ice packs

• Application Notes
• WB: 1/500-1/1000
  IF: 1/50-1/200
  IP: 1/20

• Alternative Names
• KAT9

• Gene ID
• 55140

• UniProt ID
• Q9H9T3

• Long Name
• Elongator acetyltransferase complex subunit 3

• Cellular Localization
• Cytoplasm, Nucleus

• Post Translation Modifications
• Tyrosine-phosphorylated; phosphorylation on Tyr-202 does not affect elongator complex integrity or ELP3 protein stability (PubMed:31341009).
  Also serine/threonine-phosphorylated (PubMed:31341009).
  (Q9H9T3-ELP3_HUMAN)
  Ubiquitination at Lys34, Lys48, Lys206, Lys333, Lys338, and Lys392 (NX_Q9H9T3 [NX_Q9H9T3-1])
  Modification sites at PhosphoSitePlus (Q9H9T3)
  Glycosylation from GlyGen (Q9H9T3) 1 site, 1 O-linked glycan (1 site)

• Protein Refseq
• XP_024302952.1; XP_054216724.1

• Function
• Catalytic tRNA acetyltransferase subunit of the elongator complex which is required for multiple tRNA modifications, including mcm5U (5-methoxycarbonylmethyl uridine), mcm5s2U (5-methoxycarbonylmethyl-2-thiouridine), and ncm5U (5-carbamoylmethyl uridine) (PubMed:29415125).
  In the elongator complex, acts as a tRNA uridine(34) acetyltransferase by mediating formation of carboxymethyluridine in the wobble base at position 34 in tRNAs (By similarity).
  May also act as a protein lysine acetyltransferase by mediating acetylation of target proteins; such activity is however unclear in vivo and recent evidences suggest that ELP3 primarily acts as a tRNA acetyltransferase.

Isotype Control

• MOB-208CQ
• Rabbit IgG Isotype Control Antibody

Recommended Dilution Buffer

• MRO-0253YJ
• Reconstitution Buffer PBS

Secondary Antibody

• 2RD-A1473-YJ
• Goat Anti-Rabbit IgG-F(ab')2 Secondary antibody