Recombinant Chimeric (Mouse/Human) Anti-gB Antibody (CK1)
CAT#: FAMAB-0242-CN
This is a chimeric mouse-human monoclonal antibody (cMAb) against BP and/or BM. The cMAb BP7 10B11 (cMAb CK1) reacts with glycoproteins (34, 38, 48 kDa in BP; 28, 38, 48 kDa in BM).
Published Data
ELISA
Figure 1 Binding property of 3 cMAbs produced by CHO-DG44 cells without DHFR amplification.
For ELISA, the pIRES-H(L)-DHFR vectors containing chimeric H-chain (H1, H2, and H3) or L-chain (L1, L2, and L3) were cotransfected into CHO-DG44 cells using a LF-2000 reagent and the master seed cells were incubated for 3 days to get sufficient amounts of cMAbs before the DHFR-amplification process.
WB
Figure 2 Western immunoblot analysis of BP, BM, and BT with 3 cMAbs.
For Western blot analysis, 3 cMAb culture supernatants (cMAbs CK1, CK2, and CK3) were used at 1:5 dilutions.
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Specifications
- Host Species
- Human
- Derivation
- Chimeric (mouse/human)
- Type
- Chimeric (mouse/human) IgG1, Κ
- Specificity
- Burkholderia pseudomallei gB
- Species Reactivity
- B. pseudomallei, B. mallei
- Clone
- CK1
- Applications
- Used for immunoassay techniques such as: Western Blotting; Enzyme Linked Immunoabsorbent Assay
Product Property
- Storage
- Store at -20°C for long-term storage. Avoid freeze/thaw cycles.
Target
- Alternative Names
- gB
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Easy to Handle
This antibody is simple to handle. Clear instructions are provided, and the antibody holds up well throughout several tests.
Flexible Applications
With excellent results, we have employed this antibody in a number of applications, such as immunofluorescence and immunoprecipitation.
Increased Shelf Life
This antibody is a cost-effective choice for our lab because of its extended shelf life. No problems with deterioration.
Q&As
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Is it allowed to utilize a secondary antibody from another supplier?
A: When using secondary antibodies, ensure compatibility by matching the host species and subclass of the primary antibody.
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What should I do if my antibody displays nonspecific binding?
A: To minimize non-specific binding, optimize blocking conditions, antibody concentrations, and washing protocols.
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How do I avoid antibody aggregation?
A: Incorporate stabilizers like glycerol or BSA, store at recommended temperatures, and avoid repeated freeze-thaw cycles to prevent antibody aggregation.
View the frequently asked questions answered by Creative Biolabs Support.
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Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
See other products for "Clone CK1"
- CAT
- Product Name
See other products for "gB"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PSBL-114 | Recombinant Human Anti-HCMV glycoprotein B Antibody scFv Fragment (1G2 ) | WB, ELISA, Neut, FuncS | scFv |
| PSBL-115 | Recombinant Human Anti-HCMV Glycoprotein B Antibody scFv Fragment (KE5) | WB, ELISA, IF, FuncS | scFv |
| PSBL-116 | Recombinant Human Anti-HCMV glycoprotein B Antibody scFv Fragment (SM5-1) | Neut, FuncS | scFv |
| FAMAB-0242-CN-S(P) | Recombinant Mouse Anti-gB Antibody scFv Fragment (CK1) | WB, ELISA | Mouse scFv |
| FAMAB-0274-CN-S(P) | Recombinant Mouse Anti-gB Antibody scFv Fragment (gB-15) | ELISA | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABZ-056 | Recombinant Human Anti-HCMV AD-2S1 epitope of gB Antibody (8F9) | ELISA, Neut, FACS | IgG |
| FAMAB-0274-CN | Recombinant Mouse Anti-gB Antibody (gB-15) | ELISA | Mouse IgG |
| FAMAB-0275-CN | Recombinant Mouse Anti-gB Antibody (gB-18) | ELISA | Mouse IgG |
| FAMAB-0276-CN | Recombinant Mouse Anti-gB Antibody (gB-14) | ELISA | Mouse IgG |
| FAMAB-0277-CN | Recombinant Human Anti-gB Antibody (gB-17) | ELISA | Human IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PFBZ-056 | Recombinant Human Anti-HCMV AD-2S1 epitope of gB Antibody Fab Fragment (8F9) | ELISA, Neut, FACS | |
| PFBW-056 | Recombinant Human Anti-HCMV AD-2S1 epitope of gB Antibody Fab Fragment (M2J1) | ELISA, Neut, FACS | |
| PFBC-057 | Recombinant Human Anti-HCMV AD-2S1 epitope of gB Antibody Fab Fragment (M2J5) | ELISA, Neut, FACS | |
| FAMAB-0117-CN-F(E) | Recombinant Mouse Anti-gB Antibody Fab Fragment (HCMV37) | IP | Mouse Fab |
| FAMAB-0242-CN-F(E) | Recombinant Chimeric (Mouse/Human) Anti-gB Antibody Fab Fragment (CK1) | WB, ELISA | Chimeric (mouse/human) Fab |
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