Recombinant Chimeric (Mouse/Human) Anti-gB Antibody (CK1) (CAT#: FAMAB-0242-CN)
This is a chimeric mouse-human monoclonal antibody (cMAb) against BP and/or BM. The cMAb BP7 10B11 (cMAb CK1) reacts with glycoproteins (34, 38, 48 kDa in BP; 28, 38, 48 kDa in BM).
Specific Inquiry
ELISA
Figure 1 Binding property of 3 cMAbs produced by CHO-DG44 cells without DHFR amplification.
For ELISA, the pIRES-H(L)-DHFR vectors containing chimeric H-chain (H1, H2, and H3) or L-chain (L1, L2, and L3) were cotransfected into CHO-DG44 cells using a LF-2000 reagent and the master seed cells were incubated for 3 days to get sufficient amounts of cMAbs before the DHFR-amplification process.
WB
Figure 2 Western immunoblot analysis of BP, BM, and BT with 3 cMAbs.
For Western blot analysis, 3 cMAb culture supernatants (cMAbs CK1, CK2, and CK3) were used at 1:5 dilutions.
Specifications
- Host Species
- Human
- Derivation
- Chimeric (mouse/human)
- Type
- Chimeric (mouse/human) IgG1, Κ
- Specificity
- Burkholderia pseudomallei gB
- Species Reactivity
- B. pseudomallei, B. mallei
- Clone
- CK1
- Applications
- Used for immunoassay techniques such as: Western Blotting; Enzyme Linked Immunoabsorbent Assay
Product Property
- Storage
- Store at -20°C for long-term storage. Avoid freeze/thaw cycles.
Target
- Alternative Names
- gB
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Customer Reviews and Q&As
Customer Review
Q&As
Easy to Handle
This antibody is simple to handle. Clear instructions are provided, and the antibody holds up well throughout several tests.
Flexible Applications
With excellent results, we have employed this antibody in a number of applications, such as immunofluorescence and immunoprecipitation.
Increased Shelf Life
This antibody is a cost-effective choice for our lab because of its extended shelf life. No problems with deterioration.
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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