Human Anti-GNB5 Recombinant Antibody (clone H9)
CAT#: VS-0323-CJ23
This human anti-GNB5 antibody (clone H9) is a product that recognizes Human guanine nucleotide-binding protein beta 5 (GBB5).
Gene Expression
Published Data
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nuclear speckles.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/44198/759_B10_2_selected.jpg
Normal Tissue
Figure 2 IHC staining of human hippocampus
Immunohistochemical staining of human hippocampus shows moderate cytoplasmic positivity in neuropil.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/41942/90632_B_9_6_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Neuronal cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/41942/90632_B_8_5.jpg
Normal Tissue
Figure 4 Colon
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/41942/90632_A_7_3.jpg
Normal Tissue
Figure 5 Kidney
Cells in glomeruli Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous Cells in tubules Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/41942/90632_A_9_5.jpg
Normal Tissue
Figure 6 Testis
Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/41942/90632_A_6_6.jpg
RNA Expression
Figure 7 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000069966-GNB5
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ELISA
Figure 1 ELISA of scFv-displayed virions against the GBB5 peptide.
ScFv antibodies, raised against the GBB5 peptide, are displayed on phage particles and used to probe peptides, which are captured on microtiter plate well surface.
ELISA
Figure 2 Five scFvs in a soluble ELISA retain their specificity.
After transferring the scFv coding region from the phagemid into an expression vector, these five scFvs were expressed in soluble form and purified. To demonstrate the specificity of each antibody, the ELISA was performed against all biomarker peptides available.
IA
Figure 3 Photonic crystal binding curve.
The anti-GBB5-scFv H9 was titrated across wells of a streptavidincoated, photonic crystal, 96-well, biosensor plate, with the GBB5 peptide immobilized on the well surface. The half maximal effective concentration (EC50) of ~ 300 nM was used to
estimate affinity.
WB
Figure 4 Western blot of retinal lysates.
The anti-GBB5-scFv H9 was used to probe retinal lysates from chicken, mouse, and rabbit. The left panel is probed with the scFv antibody and the secondary anti-FLAG-HRP antibody. The right panel serves as the negative control (i.e., probed with the secondary antibody only).
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Specifications
- Host Species
- Human
- Type
- Human IgG
- Specificity
- Human guanine nucleotide-binding protein beta 5 (GBB5)
- Species Reactivity
- Human
- Clone
- H9
- Applications
- Enzyme-linked Immunosorbent Assay, Western Blot, Immunoassay
- Conjugate
- Unconjugated
- Related Disease
- Retinal injury
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- PBS
- Storage
- Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody against the retinal protein, GBB5, had a dissociation constant ~300 nM and recognized the full-length endogenous protein in retinal homogenates of three different animal species by western blot. Its dimeric reagent yielded a 25-fold lower detection limit in western blot (1 μg/mL).
Target
- Alternative Names
- GB5; HG2E; IDDCA; LADCI; gbeta5
- Gene ID
- 10681
- UniProt ID
- O14775
- Sequence Similarities
- Belongs to the WD repeat G protein beta family.
- Cellular Localization
- Membrane
- Protein Refseq
- NP_006569.1; NP_057278.2
- Function
- Enhances GTPase-activating protein (GAP) activity of regulator of G protein signaling (RGS) proteins, hence involved in the termination of the signaling initiated by the G protein coupled receptors (GPCRs) by accelerating the GTP hydrolysis on the G-alpha subunits, thereby promoting their inactivation (Probable). Increases RGS9 GTPase-activating protein (GAP) activity, hence contributes to the deactivation of G protein signaling initiated by D2 dopamine receptors (PubMed:27677260).
May play an important role in neuronal signaling, including in the parasympathetic, but not sympathetic, control of heart rate (By similarity).
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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| CAT | Product Name | Application | Type |
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| MOB-1743MZ | Recombinant Mouse Anti-Human GNB5 Antibody | WB | Mouse antibody |
| VS-0323-CJ24 | Human Anti-GNB5 Recombinant Antibody (clone A1) | ELISA, WB | Human IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC311 | AbPlus™ Anti-GNB5 Magnetic Beads (VS-0724-YC311) | IP, Protein Purification |
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