Human Anti-GNB5 Recombinant Antibody (clone H9) (CAT#: VS-0323-CJ23)
This human anti-GNB5 antibody (clone H9) is a product that recognizes Human guanine nucleotide-binding protein beta 5 (GBB5).
Specific Inquiry
ELISA
Figure 1 ELISA of scFv-displayed virions against the GBB5 peptide.
ScFv antibodies, raised against the GBB5 peptide, are displayed on phage particles and used to probe peptides, which are captured on microtiter plate well surface.
ELISA
Figure 2 Five scFvs in a soluble ELISA retain their specificity.
After transferring the scFv coding region from the phagemid into an expression vector, these five scFvs were expressed in soluble form and purified. To demonstrate the specificity of each antibody, the ELISA was performed against all biomarker peptides available.
IA
Figure 3 Photonic crystal binding curve.
The anti-GBB5-scFv H9 was titrated across wells of a streptavidincoated, photonic crystal, 96-well, biosensor plate, with the GBB5 peptide immobilized on the well surface. The half maximal effective concentration (EC50) of ~ 300 nM was used to
estimate affinity.
WB
Figure 4 Western blot of retinal lysates.
The anti-GBB5-scFv H9 was used to probe retinal lysates from chicken, mouse, and rabbit. The left panel is probed with the scFv antibody and the secondary anti-FLAG-HRP antibody. The right panel serves as the negative control (i.e., probed with the secondary antibody only).
Specifications
- Host Species
- Human
- Type
- Human IgG
- Specificity
- Human guanine nucleotide-binding protein beta 5 (GBB5)
- Species Reactivity
- Human
- Clone
- H9
- Applications
- Enzyme-linked Immunosorbent Assay, Western Blot, Immunoassay
- Conjugate
- Unconjugated
- Related Disease
- Retinal injury
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- PBS
- Storage
- Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody against the retinal protein, GBB5, had a dissociation constant ~300 nM and recognized the full-length endogenous protein in retinal homogenates of three different animal species by western blot. Its dimeric reagent yielded a 25-fold lower detection limit in western blot (1 μg/mL).
Target
- Alternative Names
- GB5; HG2E; IDDCA; LADCI; gbeta5
- Gene ID
- 10681
- UniProt ID
- O14775
- Sequence Similarities
- Belongs to the WD repeat G protein beta family.
- Cellular Localization
- Membrane
- Protein Refseq
- NP_006569.1; NP_057278.2
- Function
- Enhances GTPase-activating protein (GAP) activity of regulator of G protein signaling (RGS) proteins, hence involved in the termination of the signaling initiated by the G protein coupled receptors (GPCRs) by accelerating the GTP hydrolysis on the G-alpha subunits, thereby promoting their inactivation (Probable). Increases RGS9 GTPase-activating protein (GAP) activity, hence contributes to the deactivation of G protein signaling initiated by D2 dopamine receptors (PubMed:27677260).
May play an important role in neuronal signaling, including in the parasympathetic, but not sympathetic, control of heart rate (By similarity).
Recommended Products
Isotype Control
Secondary Antibody
Recommended Dilution Buffer
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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See other products for "GNB5"
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1743MZ | Recombinant Mouse Anti-Human GNB5 Antibody | WB | Mouse antibody |
| VS-0323-CJ24 | Human Anti-GNB5 Recombinant Antibody (clone A1) | ELISA, WB | Human IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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