Human Anti-GPRV Recombinant Antibody (clone IV-67) (CAT#: FAMAB-0163WJ)

Figure 1 Western blot analysis showing the position of the scFv-Fc fusion protein.

Lane 1, Molecular weight markers (97.4 kD-21.5 kD); Lane 2, III-20-Fc; Lane 3, IV-67-Fc.

Ray, K., Embleton, M. J., Jailkhani, B. L., Bhan, M. K., & Kumar, R. (2001). Selection of single chain variable fragments (scFv) against the glycoprotein antigen of the rabies virus from a human synthetic scFv phage display library and their fusion with the Fc region of human IgG1. Clinical and experimental immunology, 125(1), 94–101.

Figure 2 Dot blot analysis showing the specific binding of fusion proteins to GPRV and PV11.

These were detected with III-20-Fc (lane 1), IV-67-Fc (lane 2), Fc (lane 3, negative control) and HRIG (lane 4, positive control) followed by HRP conjugated anti-human IgG.

Ray, K., Embleton, M. J., Jailkhani, B. L., Bhan, M. K., & Kumar, R. (2001). Selection of single chain variable fragments (scFv) against the glycoprotein antigen of the rabies virus from a human synthetic scFv phage display library and their fusion with the Fc region of human IgG1. Clinical and experimental immunology, 125(1), 94–101.

Figure 3 Membrane immunofluorescence of PV11 infected Vero cells with (a) III-20-Fc, (b) IV-67-Fc and (c) HRIG.

Magnifications indicated are on the film. Live single cell suspensions of PV11 infected Vero cells were treated with either the fusion proteins, or HRIG as primary antibodies followed by FITC conjugated anti-human IgG.

Ray, K., Embleton, M. J., Jailkhani, B. L., Bhan, M. K., & Kumar, R. (2001). Selection of single chain variable fragments (scFv) against the glycoprotein antigen of the rabies virus from a human synthetic scFv phage display library and their fusion with the Fc region of human IgG1. Clinical and experimental immunology, 125(1), 94–101.