Human Anti-GXM Recombinant Antibody (clone 2E9) (CAT#: FAMAB-0573WJ)
This product is a human monoclonal antibody that is specific for C. neoformans GXM. This antibody can be used in a variety of applications, such as ELISA, WB, IF, Neut.
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
Figure 1 Binding of ELISA-selected phages to 2E9.
The phages shown were from the 60-min ELISA-selected library (Table 2). ELISA plates coated with MAb 2E9 were incubated with serial dilutions of the phage particles, and binding was detected with a biotinylated sheep anti-M13 antibody. MAb 2E9 binding for each dilution of phage depicted on the x axis is plotted on the y axis as the A405. Phage 33 lacks a peptide insert, and P-N is an ELISA-selected phage that did not produce an ECL signal.
Zhang, H., Zhong, Z., & Pirofski, L. A. (1997). Peptide epitopes recognized by a human anti-cryptococcal glucuronoxylomannan antibody. Infection and immunity, 65(4), 1158-1164.
Figure 2 Phage inhibition of the GXM binding of human 2E9.
The phages shown were from the 60-min and the second-round bead ELISA selected libraries. Phage 4-27 is the same as phage 1 from the third-round bead ELISA-selected library. ELISA plates coated with GXM were incubated with MAb 2E9 alone and with mixtures of serial dilutions of the phage (in quadruplicate) with 1 μg of 2E9 per ml. Binding of 2E9 was detected with alkaline phosphatase-labeled goat anti-human IgM). The phage concentration that inhibited 50% of 2E9 binding is plotted on the y axis for each of the phages designated on the x axis. The parentheses used to designate some of the phage represent the library from which that phage was isolated. The inhibitory concentrations of phages 2-26, 9,21, 13, 10-23, 10(2nd), 9 (2nd), and 8 (2nd) were all greater than that of phage 4-27, and the inhibitory concentrations of phages 9 (2nd) and 8(2nd) were greater than that of phage 10 (2nd).
Zhang, H., Zhong, Z., & Pirofski, L. A. (1997). Peptide epitopes recognized by a human anti-cryptococcal glucuronoxylomannan antibody. Infection and immunity, 65(4), 1158-1164.
Figure 3 Peptide 13 reactivity of human IgM antibodies.
MAb 2E9 and two other IgM MAbs were incubated with peptide 13-coated ELISA plates, and antibody binding was detected as described in the text. The absorbances of the MAbs are shown on the y axis for each antibody concentration shown on the x axis.
Zhang, H., Zhong, Z., & Pirofski, L. A. (1997). Peptide epitopes recognized by a human anti-cryptococcal glucuronoxylomannan antibody. Infection and immunity, 65(4), 1158-1164.
Specifications
- Host Species
- Human
- Type
- Human IgG
- Specificity
- C. neoformans GXM
- Species Reactivity
- C. neoformans
- Clone
- 2E9
- Applications
- ELISA, WB, IF, Neut
- Related Disease
- C. neoformans infection
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
- Alternative Names
- C. neoformans GXM; GXM
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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See other products for "GXM"
Select a product category from the dropdown menu below to view related products.
CAT | Product Name | Application | Type |
---|---|---|---|
FAMAB-0062-CN | Human Anti-GXM Recombinant Antibody (clone G19) | FC, Inhib | Human IgM |
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