Human Anti-GXM Recombinant Antibody (clone 2E9) (CAT#: FAMAB-0573WJ)

Figure 1 Binding of ELISA-selected phages to 2E9.

The phages shown were from the 60-min ELISA-selected library (Table 2). ELISA plates coated with MAb 2E9 were incubated with serial dilutions of the phage particles, and binding was detected with a biotinylated sheep anti-M13 antibody. MAb 2E9 binding for each dilution of phage depicted on the x axis is plotted on the y axis as the A405. Phage 33 lacks a peptide insert, and P-N is an ELISA-selected phage that did not produce an ECL signal.

Zhang, H., Zhong, Z., & Pirofski, L. A. (1997). Peptide epitopes recognized by a human anti-cryptococcal glucuronoxylomannan antibody. Infection and immunity, 65(4), 1158-1164.

Figure 2 Phage inhibition of the GXM binding of human 2E9.

The phages shown were from the 60-min and the second-round bead ELISA selected libraries. Phage 4-27 is the same as phage 1 from the third-round bead ELISA-selected library. ELISA plates coated with GXM were incubated with MAb 2E9 alone and with mixtures of serial dilutions of the phage (in quadruplicate) with 1 μg of 2E9 per ml. Binding of 2E9 was detected with alkaline phosphatase-labeled goat anti-human IgM). The phage concentration that inhibited 50% of 2E9 binding is plotted on the y axis for each of the phages designated on the x axis. The parentheses used to designate some of the phage represent the library from which that phage was isolated. The inhibitory concentrations of phages 2-26, 9,21, 13, 10-23, 10(2nd), 9 (2nd), and 8 (2nd) were all greater than that of phage 4-27, and the inhibitory concentrations of phages 9 (2nd) and 8(2nd) were greater than that of phage 10 (2nd).

Zhang, H., Zhong, Z., & Pirofski, L. A. (1997). Peptide epitopes recognized by a human anti-cryptococcal glucuronoxylomannan antibody. Infection and immunity, 65(4), 1158-1164.

Figure 3 Peptide 13 reactivity of human IgM antibodies.

MAb 2E9 and two other IgM MAbs were incubated with peptide 13-coated ELISA plates, and antibody binding was detected as described in the text. The absorbances of the MAbs are shown on the y axis for each antibody concentration shown on the x axis.

Zhang, H., Zhong, Z., & Pirofski, L. A. (1997). Peptide epitopes recognized by a human anti-cryptococcal glucuronoxylomannan antibody. Infection and immunity, 65(4), 1158-1164.