Human Anti-HIV-1 gp120 Recombinant Antibody (clone DH374) (CAT#: MRO-3082CQ)

Figure 1 HIV-1 gp120-specific colostrum monoclonal antibodies (mAbs) are crossreactive with commensal bacteria whole-cell lysate (WCL) by western blot, including specific reactivity against Escherichia coli Chaperonin 60.

The specificity of DH374 for E. coli Chaperonin 60 was confirmed by both reduced and nonreduced SDSPAGE western blot.

Jeffries, T. L., Sacha, C. R., Pollara, J., Himes, J., Jaeger, F. H., Dennison, S. M., ... & LaBranche, C. (2016). The function and affinity maturation of HIV-1 gp120-specific monoclonal antibodies derived from colostral B cells. Mucosal immunology, 9(2), 414-427.

Figure 2 HIV-1 gp120-specific colostrum monoclonal antibodies (mAbs) are crossreactive with commensal bacteria whole-cell lysate (WCL) by western blot, including specific reactivity against Escherichia coli Chaperonin 60.

DH374 binding to Chaperonin 60 was also confirmed by binding enzyme-linked immunosorbent assay (ELISA) to the nonreduced E. coli Chaperonin 60 protein (three fold dilutions, ranging from 100 to 0.01 mg/ml). DH375, a noncommensal bacteria-reactive colostrum mAb, was used as a negative control. OD, optical density.

Jeffries, T. L., Sacha, C. R., Pollara, J., Himes, J., Jaeger, F. H., Dennison, S. M., ... & LaBranche, C. (2016). The function and affinity maturation of HIV-1 gp120-specific monoclonal antibodies derived from colostral B cells. Mucosal immunology, 9(2), 414-427.

Figure 3 Inhibition of HIV-1 epithelial cell binding and dendritic cell (DC)-mediated trans-infection by envelope (Env)-specific colostrum monoclonal antibodies (mAbs).

Of the 15 colostrum Env-specific mAbs, 11 inhibit epithelial cell binding of the clade C HIV-1 variant MW965. Epithelial cell binding inhibition was mediated at a high level by the HIV-1 MW965-neutralizing mAbs (red bars), and at a low level by one nonneutralizing gp120-specific mAb (DH390) and gp41 mAbs (DH288 and DH389).

Jeffries, T. L., Sacha, C. R., Pollara, J., Himes, J., Jaeger, F. H., Dennison, S. M., ... & LaBranche, C. (2016). The function and affinity maturation of HIV-1 gp120-specific monoclonal antibodies derived from colostral B cells. Mucosal immunology, 9(2), 414-427.