Mouse Anti-HIV-1 gp41 Recombinant Antibody (clone T26) (CAT#: MRO-4181CQ)

Recombinant mouse antibody to gp41. T26 was found to predominantly bind to oligomeric gp41 and not to monomeric gp41. Binding of this Ab to H9/IIIB-infected cells gave a weak signal which was slightly increased by sCD4 pretreatment.

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WB

Figure 1 WB analysis of mAbs. Each WB strip was incubated with 20 µl sera,
32 µg T26 mAb or 4 µg other mAbs diluted with 2 ml wash solution.

Figure 1 WB analysis of mAbs. Each WB strip was incubated with 20 µl sera, 32 µg T26 mAb or 4 µg other mAbs diluted with 2 ml wash solution.

The
healthy volunteer sera as negative control and AIDS patient sera as positive
control reference were performed.

Guenaga, J., Dosenovic, P., Ofek, G., Baker, D., Schief, W. R., Kwong, P. D., ... & Wyatt, R. T. (2011). Heterologous Epitope-Scaffold Prime∶ Boosting Immuno-Focuses B Cell Responses to the HIV-1 gp41 2F5 Neutralization Determinant. PloS one, 6(1).

FC

Figure 2 Properties of mAbs binding to H9/IIIB or MN. Effect of sCD4 to env
protein on H9/IIIB.

Figure 2 Properties of mAbs binding to H9/IIIB or MN. Effect of sCD4 to env protein on H9/IIIB.

Each bar ( ± S.E.M.) presents the
mean of triplicate determinations.

Usami, O., Xiao, P., Ling, H., Liu, Y., Nakasone, T., & Hattori, T. (2005). Properties of anti-gp41 core structure antibodies, which compete with sera of HIV-1-infected patients. Microbes and infection, 7(4), 650-657.

Inhib

Figure 3 Inhibition of sera against binding of mAbs 50.69, 98.6, T26 and 902.

Figure 3 Inhibition of sera against binding of mAbs 50.69, 98.6, T26 and 902.

The average of competition between pre-bound sera and mAbs.

Usami, O., Xiao, P., Ling, H., Liu, Y., Nakasone, T., & Hattori, T. (2005). Properties of anti-gp41 core structure antibodies, which compete with sera of HIV-1-infected patients. Microbes and infection, 7(4), 650-657.

FuncS

Figure 4 The temperature dependency of MAb T26 binding.

Figure 4 The temperature dependency of MAb T26 binding.

MAb T26 binding to H9/IIIB at 37 C was higher than at 4 C.

Usami, O., Xiao, P., Ling, H., & Hattori, T. (2006). Competitive Study of Monoclonal Antibodies against the HIV‐1 Gp41 Core Structure. Microbiology and immunology, 50(2), 131-134.

Inhib

Figure 5 The decreased binding of biotinylated MAb 50.69, 98.6, or T26 to H9/IIIB cells after preincubation with MAb 50.69 (open bars), 98.6 (filled bars), or hIgG (gray bars).

Figure 5 The decreased binding of biotinylated MAb 50.69, 98.6, or T26 to H9/IIIB cells after preincubation with MAb 50.69 (open bars), 98.6 (filled bars), or hIgG (gray bars).

The negative control was calculated as the MFI without preincubation MAb and with biotinylated MAb.

Usami, O., Xiao, P., Ling, H., & Hattori, T. (2006). Competitive Study of Monoclonal Antibodies against the HIV‐1 Gp41 Core Structure. Microbiology and immunology, 50(2), 131-134.


Specifications

  • Host Species
  • Mouse
  • Type
  • Mouse IgG
  • Specificity
  • Recognizes HIV-1 gp41
  • Species Reactivity
  • HIV-1
  • Clone
  • T26
  • Applications
  • Inhib, FC, WB, FuncS

Product Property

  • Purity
  • >95% as determined by SDS-PAGE
  • Concentration
  • Please refer to the vial label for the specific concentration.
  • Buffer
  • PBS
  • Preservative
  • No preservatives
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

  • Application Notes
  • The HIV-1 gp41 antibody has been reported in applications of Inhibition, Flow Cytometry, Western blot, Functional Assay. FC: Cells were incubated with
    32 µg/ml mAb T26 and 4 µg/ml other mAbs, followed by FITC-aH or -aM
    incubation.

Target

  • Alternative Names
  • Human immunodeficiency virus type 1; HIV-1; Envelope glycoprotein GP41; gp41; HIV envelope

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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