Mouse Anti-IgL Recombinant Antibody (clone S.46) (CAT#: FAMAB-0028-CN)

The anti-catfish IgL S.46 antibody specifically reacts with the denatured form of catfish IgL σ. This anti-catfish IgL σ mAb may be useful in studies examining the role of catfish IgL σ chain expression in immune responses.


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WB

Figure 1 Western blot showing IgL chain sizes in catfish serum.

Figure 1 Western blot showing IgL chain sizes in catfish serum.

Onemicroliter serum samples from fish #23 were separated by 12% SDS-PAGE under reducing conditions, transferred to a nitrocellulose membrane, and visualized by Western blot using anti-IgL σ S.46 mAb, anti-IgL F mAb, or anti-IgL G mAb. Arrows indicate IgL σ-, IgL F, and IgL G chains.

Edholm, E. S., Bengtén, E., Stafford, J. L., Sahoo, M., Taylor, E. B., Miller, N. W., & Wilson, M. (2010). Identification of two IgD+ B cell populations in channel catfish, Ictalurus punctatus. The Journal of Immunology, 185(7), 4082-4094.


Specifications

  • Immunogen
  • Recombinant IgL σ made in a prokaryotic expression system
  • Host Species
  • Mouse
  • Type
  • Mouse IgG2b, κ
  • Specificity
  • Catfish IgL
  • Species Reactivity
  • Catfish
  • Clone
  • S.46
  • Applications
  • WB, ELISA, FC

Product Property

  • Purity
  • >95% as determined by SDS-PAGE and HPLC analysis
  • Concentration
  • Please refer to the vial label for the specific concentration.
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

  • Application Notes
  • This antibody has been reported for use in Western Blot, Enzyme-Linked Immunosorbent Assay and Flow Cytometry.
    WB: immunoselected proteins were separated by 10% SDS-PAGE gel and visualized in Western blot using anti-catfish IgL σ mAb S.46 and HRP-conjugated goat anti-mouse IgG2b diluted 1/5000 (v/v).

Target

  • Alternative Names
  • IgL

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

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Recombinant Antibody

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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