Mouse Anti-IgL Recombinant Antibody (clone 3F12) (CAT#: FAMAB-0034-CN)
The monoclonal antibody 3F12 reacts with catfish IgL F isotype. This antibody has specificities for the catfish equivalents of kappa and lambda chains.
Specific Inquiry
FC
Figure 1 CC41+ cells possess a putative FcμR.
PBL were isolated from a G14D-CCV immunized fish 3 d after a booster immunization. An aliquot of these cells was analyzed for IgL chain staining with anti-IgL F (3F12) and IgL G (11A2) mAbs, followed by goat anti-mouse IgG1 FITC and goat anti-mouse IgG2b RPE. The remainder of the cells were sequentially magnetically sorted with 3F12 and 11A2 mAbs and separated into IgL F−/IgL G−, IgL F+ or IgL G+, and IgL F+ and IgL G+ cell populations. Each cell population was then stained with CC41 mAb directly conjugated to Alexa Fluor 488. Percentages of positive cells are indicated in the upper right corner, and staining with the isotype control Ab 1.14 is outlined in gray.
Taylor, E. B., Moulana, M., Stuge, T. B., Quiniou, S. M., Bengten, E., & Wilson, M. (2016). A leukocyte immune-type receptor subset is a marker of antiviral cytotoxic cells in channel catfish, Ictalurus punctatus. The Journal of Immunology, 196(6), 2677-2689.
Specifications
- Immunogen
- purified catfish immunoglobulin
- Host Species
- Mouse
- Type
- Mouse IgG1, κ
- Specificity
- Catfish IgL
- Species Reactivity
- Catfish
- Clone
- 3F12
- Applications
- IF, FC
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been reported for use in Immunofluorescence and Flow Cytometry.
FC: PBL were isolated from G14D and G14D-CCV–immunized fish at the indicated time points, resuspended at 1×10⁷ cells/ml in cfRPMI, and analyzed for CC41 staining using flow cytometry analysis. For single-color flow cytometry, 1×10⁶ cells were incubated with 50 ml mAb supernatant for 30 min on ice, washed with cfRPMI, and incubated with 50 ml goat anti-mouse IgG1-PE (1:80 v/v in cfRPMI) for 30 min on ice. After washing, samples were resuspended in 500 ml cfRPMI and analyzed using the FACScan. The 3F12⁺ /11A2⁺, 3F12⁺ /11A22⁺ 3F122/11A2⁺, and 3F122/11A22 cell populations were analyzed as above.
Target
- Alternative Names
- IgL
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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See other products for "Clone 3F12"
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Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1385z | Mouse Anti-IGL Recombinant Antibody (clone 43C2) | FC, IF, IHC, WB | Mouse IgG2a, κ |
| FAMAB-0028-CN | Mouse Anti-IgL Recombinant Antibody (clone S.46) | WB, ELISA, FC | Mouse IgG2b, κ |
| FAMAB-0029-CN | Mouse Anti-IgL Recombinant Antibody (clone S.4) | WB, ELISA, FC | Mouse IgG3, κ |
| FAMAB-0030-CN | Mouse Anti-IgL Recombinant Antibody (clone S.28) | WB, ELISA, FC | Mouse IgG3, κ |
| FAMAB-0035-CN | Mouse Anti-IgL Recombinant Antibody (clone 11A2) | WB, IP, FC | Mouse IgG2b, κ |
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0028-CN-S(P) | Mouse Anti-IgL Recombinant Antibody (clone S.46); scFv Fragment | WB, ELISA, FC | Mouse scFv |
| FAMAB-0029-CN-S(P) | Mouse Anti-IgL Recombinant Antibody (clone S.4); scFv Fragment | WB, ELISA, FC | Mouse scFv |
| FAMAB-0030-CN-S(P) | Mouse Anti-IgL Recombinant Antibody (clone S.28); scFv Fragment | WB, ELISA, FC | Mouse scFv |
| FAMAB-0034-CN-S(P) | Mouse Anti-IgL Recombinant Antibody (clone 3F12); scFv Fragment | IF | Mouse scFv |
| FAMAB-0035-CN-S(P) | Mouse Anti-IgL Recombinant Antibody (clone 11A2); scFv Fragment | WB, IP, FC | Mouse scFv |
Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0028-CN-F(E) | Mouse Anti-IgL Recombinant Antibody (clone S.46); Fab Fragment | WB, ELISA, FC | Mouse Fab |
| FAMAB-0029-CN-F(E) | Mouse Anti-IgL Recombinant Antibody (clone S.4); Fab Fragment | WB, ELISA, FC | Mouse Fab |
| FAMAB-0030-CN-F(E) | Mouse Anti-IgL Recombinant Antibody (clone S.28); Fab Fragment | WB, ELISA, FC | Mouse Fab |
| FAMAB-0034-CN-F(E) | Mouse Anti-IgL Recombinant Antibody (clone 3F12); Fab Fragment | IF | Mouse Fab |
| FAMAB-0035-CN-F(E) | Mouse Anti-IgL Recombinant Antibody (clone 11A2); Fab Fragment | WB, IP, FC | Mouse Fab |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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