Mouse Anti-IgL Recombinant Antibody (clone 3F12) (CAT#: FAMAB-0034-CN)

• Immunogen
• purified catfish immunoglobulin

• Host Species
• Mouse

• Type
• Mouse IgG1, κ

• Specificity
• Catfish IgL

• Species Reactivity
• Catfish

• Clone
• 3F12

• Applications
• IF, FC

• Purity
• >95% as determined by SDS-PAGE and HPLC analysis

• Concentration
• Please refer to the vial label for the specific concentration.

• Storage
• Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

• Application Notes
• This antibody has been reported for use in Immunofluorescence and Flow Cytometry.
  FC: PBL were isolated from G14D and G14D-CCV–immunized fish at the indicated time points, resuspended at 1×10⁷ cells/ml in cfRPMI, and analyzed for CC41 staining using flow cytometry analysis. For single-color flow cytometry, 1×10⁶ cells were incubated with 50 ml mAb supernatant for 30 min on ice, washed with cfRPMI, and incubated with 50 ml goat anti-mouse IgG1-PE (1:80 v/v in cfRPMI) for 30 min on ice. After washing, samples were resuspended in 500 ml cfRPMI and analyzed using the FACScan. The 3F12⁺ /11A2⁺, 3F12⁺ /11A22⁺ 3F122/11A2⁺, and 3F122/11A22 cell populations were analyzed as above.

• Alternative Names
• IgL

• FAMAB-0034-CN-S(P)
• Mouse Anti-IgL Recombinant Antibody (clone 3F12); scFv Fragment

• FAMAB-0034-CN-F(E)
• Mouse Anti-IgL Recombinant Antibody (clone 3F12); Fab Fragment

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