Human Anti-K. pneumoniae MrkA Recombinant Antibody (clone Kp3) (CAT#: HPAB-2421LY)
This product is a recombinant human antibody Kp3 that recognizes K. pneumoniae MrkA.
Specific Inquiry
ELISA
Figure 1 Monoclonal antibodies isolated through phage and hybridoma platforms display K. pneumoniae binding and potent OPK activity.
Biotin-labeled hybridoma IgG2a (88D10 and 89E10) and phage antibodies IgG1 (KP3 and KP16) bind to K. pneumoniae strain 29011 in ELISA assays. Streptavidin-HRP was used as the detecting agent.
Wang, Q., Chang, C. S., Pennini, M., Pelletier, M., Rajan, S., Zha, J.,... & Yu Lin, H. (2016). Target-agnostic identification of functional monoclonal antibodies against Klebsiella pneumoniae multimeric MrkA fimbrial subunit. The Journal of infectious diseases, 213(11), 1800-1808.
IF
Figure 2 Confocal microscopy showing KP3 binding to K. pneumoniae surface.
Wang, Q., Chang, C. S., Pennini, M., Pelletier, M., Rajan, S., Zha, J.,... & Yu Lin, H. (2016). Target-agnostic identification of functional monoclonal antibodies against Klebsiella pneumoniae multimeric MrkA fimbrial subunit. The Journal of infectious diseases, 213(11), 1800-1808.
WB
Figure 3 Recombinant and in vitro transcription and translation of MrkA.
Recombinant expression of MrkA. MrkA ORF of strain MGH78578 was cloned and expressed in BL21 cells. Lysates were subjected to Western blot analysis using both anti-his tag (left panel) and KP3 (right panel). Lane 1, host cell only; Lane 2, host cell transformed with empty vector; Lane 3, host cell transformed with expression vector carrying his-tagged MrkA ORF; Lane 4, lysate prepared from strain 43816DM.
Wang, Q., Chang, C. S., Pennini, M., Pelletier, M., Rajan, S., Zha, J.,... & Yu Lin, H. (2016). Target-agnostic identification of functional monoclonal antibodies against Klebsiella pneumoniae multimeric MrkA fimbrial subunit. The Journal of infectious diseases, 213(11), 1800-1808.
FuncS
Figure 4 KP3 mAb displayed protective activity in various in vivo models.
KP3 at 15 mg/kg reduced organ burden in intranasal lung infection model against Kp29011 (O1:K2). An irrelevant human IgG1 antibody was used as control.
Wang, Q., Chang, C. S., Pennini, M., Pelletier, M., Rajan, S., Zha, J.,... & Yu Lin, H. (2016). Target-agnostic identification of functional monoclonal antibodies against Klebsiella pneumoniae multimeric MrkA fimbrial subunit. The Journal of infectious diseases, 213(11), 1800-1808.
Specifications
- Host Species
- Human
- Derivation
- Humanized
- Type
- Humanized IgG
- Specificity
- K. pneumoniae MrkA
- Clone
- Kp3
- Applications
- ELISA, IF, FC, Inhib, WB, FuncS
- Conjugate
- Unconjugated, HRP, Biotin, etc
Product Property
- Purification
- Protein A/G Purified
- Purity
- >95%
- Concentration
- Batch Specific
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Enzyme-linked Immunosorbent Assay, Sandwitch Enzyme-linked Immunosorbent Assay.
Target
- Alternative Names
- Klebsiella pneumoniae MrkA; MrkA; Klebsiella pneumoniae; K. pneumoniae
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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Fab Antibody Products
IgG Antibody Products
ScFv Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-2421LY-F(E) | Human Anti-K. pneumoniae MrkA Recombinant Antibody (clone Kp3); Fab Fragment | ELISA, IF, FC, Inhib, WB, FuncS | Humanized Fab |
| HPAB-2422LY-F(E) | Human Anti-K. pneumoniae MrkA Recombinant Antibody (clone Kp16); Fab Fragment | ELISA, FC | Humanized Fab |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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