Mouse Anti-Lectin Igl1 Recombinant Antibody (clone XEhI-20) (CAT#: FAMAB-0050JF)
Recombinant human monoclonal antibody specially binds to Entamoeba histolytica Igl1. This anti-Entamoeba histolytica Igl1 antibody XEhI-20 can be potiently used in the immunoprophylaxis of amebiasis.
Specific Inquiry
WB
Figure 1 Mouse Anti-Lectin Igl1 Antibody (clone XEhI-20) in WB.
Western immunoblot analysis of human MAbs to E. histolytica. Lysates of trophozoites from the HM-1:IMSS strain were subjected to SDS-PAGE in a 7.5% gel under nonreducing conditions and transferred onto polyvinylidene difluoride membranes.
Tachibana, H., Cheng, X. J., Tsukamoto, H., & Itoh, J. (2009). Characterization of Entamoeba histolytica intermediate subunit lectin-specific human monoclonal antibodies generated in transgenic mice expressing human immunoglobulin loci. Infection and immunity, 77(1), 549-556.
FC
Figure 2 Mouse Anti-Lectin Igl1 Antibody (clone XEhI-20) in FC.
Flow cytometric analysis of E. histolytica trophozoites stained with human MAbs to Igls (black-filled histograms). Intact trophozoites were incubated with human MAbs XEhI-20, XEhI-28, XEhI-B5, and XEhI-H2, followed by FITC-conjugated goat antibody to human IgG(H+L). The control was stained only with a secondary antibody (unfilled histograms). A representative histogram for each antibody is depicted. Fluorescence levels are expressed in arbitrary units.
Tachibana, H., Cheng, X. J., Tsukamoto, H., & Itoh, J. (2009). Characterization of Entamoeba histolytica intermediate subunit lectin-specific human monoclonal antibodies generated in transgenic mice expressing human immunoglobulin loci. Infection and immunity, 77(1), 549-556.
DB
Figure 3 Mouse Anti-Lectin Igl1 Antibody (clone XEhI-20) in DB.
Reactivity of human MAbs to recombinant Igls of E. histolytica in a dot blot analysis. Two strips were stained with Coomassie brilliant blue (CBB), and other strips were treated with MAbs XEhI-H2, XEhI-28, XEhI-20, and XEhI-B5 or PBS (control). HRP-conjugated goat antibody to human IgG(H+L) was used as a secondary antibody.
Tachibana, H., Cheng, X. J., Tsukamoto, H., & Itoh, J. (2009). Characterization of Entamoeba histolytica intermediate subunit lectin-specific human monoclonal antibodies generated in transgenic mice expressing human immunoglobulin loci. Infection and immunity, 77(1), 549-556.
IF
Figure 4 Mouse Anti-Lectin Igl1 Antibody (clone XEhI-20) in IF.
Localization of Igl1 and Igl2 on trophozoites of E. histolytica HM-1:IMSS observed by confocal laser scanning microscopy. Fixed trophozoites were stained with Alexa Fluor 488-labeled XEhI-20, specific for Igl1 (green).
Tachibana, H., Cheng, X. J., Tsukamoto, H., & Itoh, J. (2009). Characterization of Entamoeba histolytica intermediate subunit lectin-specific human monoclonal antibodies generated in transgenic mice expressing human immunoglobulin loci. Infection and immunity, 77(1), 549-556.
Specifications
- Immunogen
- Igl emulsified in complete Freund's adjuvant
- Host Species
- Mouse
- Type
- Mouse IgG2, κ
- Specificity
- Entamoeba histolytica Igl1
- Species Reactivity
- E. histolytica
- Clone
- XEhI-20
- Applications
- DB, FC, IF, WB, ELISA, FuncS
- Related Disease
- Amebiasis
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
- Alternative Names
- Lectin Igl1;Lectin
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "Clone XEhI-20"
See other products for "Lectin Igl1"
Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0050JF-F(E) | Mouse Anti-Lectin Igl1 Recombinant Antibody (clone XEhI-20); Fab Fragment | DB, FC, IF, WB, ELISA, FuncS | Mouse Fab |
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0050JF-S(P) | Mouse Anti-Lectin Igl1 Recombinant Antibody (clone XEhI-20); scFv Fragment | DB, FC, IF, WB, ELISA, FuncS | Mouse scFv |
Customer Reviews and Q&As
There are currently no Customer reviews or questions for FAMAB-0050JF. Click the button above to contact us or submit your feedback about this product.
View the frequently asked questions answered by Creative Biolabs Support.
For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.