Mouse Anti-lipA Recombinant Antibody (clone 2506W0546) (CAT#: VS-0625-FY651)
The mouse anti-lipA monoclonal antibody is a highly specific reagent for use in ELISA assays. It offers excellent sensitivity for detecting the lipA protein, a lipase enzyme. This enzyme is involved in lipid hydrolysis and plays roles in various biological processes, including nutrient acquisition and pathogenesis. This antibody is ideal for researchers investigating lipid metabolism, enzyme mechanisms, and potential drug targets. Its reliable binding ensures accurate and reproducible results, facilitating deeper insights into enzymatic functions. This antibody is a valuable tool for studies in biochemistry and microbiology.
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Subcellular Location and Protein Expression
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to vesicles.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/57052/956_A5_6_selected.jpg
(Immunofluorescent staining of human cell line A-431 shows localization to vesicles.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/57052/956_A5_6_selected.jpg
Figure 2 IHC staining of human spleen
(High expression. RNA expression: 398.4 nTPM)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/57052/141734_B_9_4_val_selected.jpg
(High expression. RNA expression: 398.4 nTPM)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/57052/141734_B_9_4_val_selected.jpg
Figure 3 IHC staining of human pancreas
(Low expression. RNA expression: 21.4 nTPM)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/57052/141734_A_3_3_val_selected.jpg
(Low expression. RNA expression: 21.4 nTPM)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/57052/141734_A_3_3_val_selected.jpg
Figure 4 Cerebral cortex
(Neuropil
Staining: Low
Intensity: Moderate
Quantity: <25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/57052/141734_B_7_5.jpg
(Neuropil
Staining: Low
Intensity: Moderate
Quantity: <25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/57052/141734_B_7_5.jpg
Figure 5 Colon
(Mucosal lymphoid cells
Staining: Medium
Intensity: Strong
Quantity: <25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/57052/141734_A_9_3.jpg
(Mucosal lymphoid cells
Staining: Medium
Intensity: Strong
Quantity: <25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/57052/141734_A_9_3.jpg
Figure 6 Liver
(Collecting ducts
Staining: Low
Intensity: Moderate
Quantity: <25%
Distal tubules
Staining: Low
Intensity: Moderate
Quantity: <25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/57052/141734_A_8_4.jpg
(Collecting ducts
Staining: Low
Intensity: Moderate
Quantity: <25%
Distal tubules
Staining: Low
Intensity: Moderate
Quantity: <25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/57052/141734_A_8_4.jpg
Figure 7 Kidney
(Collecting ducts
Staining: Low
Intensity: Moderate
Quantity: <25%
Distal tubules
Staining: Low
Intensity: Moderate
Quantity: <25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/57052/141734_A_8_5.jpg
(Collecting ducts
Staining: Low
Intensity: Moderate
Quantity: <25%
Distal tubules
Staining: Low
Intensity: Moderate
Quantity: <25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/57052/141734_A_8_5.jpg
Figure 8 Testis
(Cells in seminiferous ducts
Staining: Not detected
Intensity: Weak
Quantity: <25%
Location: Cytoplasmic/membranous
Leydig cells
Staining: Not detected
Intensity: Weak
Quantity: <25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/57052/141734_A_6_6.jpg
(Cells in seminiferous ducts
Staining: Not detected
Intensity: Weak
Quantity: <25%
Location: Cytoplasmic/membranous
Leydig cells
Staining: Not detected
Intensity: Weak
Quantity: <25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/57052/141734_A_6_6.jpg
Figure 9 Lymph node
(Non-germinal center cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/57052/141734_A_9_8.jpg
(Non-germinal center cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/57052/141734_A_9_8.jpg
Figure 10 RNA cell line category: Cell line enhanced (A-431, AN3-CA, ASC TERT1)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000107798-LIPA
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000107798-LIPA
Specifications
- Immunogen
- Pseudomonas cepacia lipA
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- The antibody specifically recognizes the peptide of the Pseudomonas cepacia lipA.
- Species Reactivity
- Pseudomonas cepacia
- Clone
- 2506W0546
- Applications
- ELISA
Product Property
- Purification
- Purified with Protein A or G affinity chromatography
- Purity
- >90% as determined by SDS-PAGE
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- The buffer commonly used is typically composed of 1 M Tris-HCl and 100 mM glycine. For more detailed information, refer to the Certificate of Analysis (COA).
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- ELISA: 1:1000-1:3000
Other applications are under development. Optimal dilutions for assays should be determined by the user.
Target
Recommended Products
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "LIPA"
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IgG Antibodies
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2590z | Mouse Anti-LIPA Recombinant Antibody (clone 30F7) | WB, IF, IHC, IP | Mouse IgG1, κ |
| MOB-1027MZ | Recombinant Mouse Anti-Human LIPA Antibody (clone 0H8G23) | ELISA, IHC-P, WB | Mouse antibody |
| ZG-0260C | Mouse Anti-LIPA Recombinant Antibody (ZG-0260C) | WB, ELISA | Mouse IgG |
| VS7-0425-WR278 | Mouse Anti-LIPA Monoclonal Antibody (clone 9G7F12) | WB, ELISA | Mouse IgG2a |
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For Research Use Only. Not For Clinical Use.
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