Recombinant Llama Anti-MARV NP Single Domain Antibody (PABC-560) (CAT#: PABC-560)
This product is a recombinant Llama antibody that can recognize MARV NP.
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IF
Figure 1 HEK293T cells were transiently transfected with various NP expression plasmids indicated at the top of the panels.
At 24 h post-transfection, the cells were probed with 10 nM of the various sdAb-APEX2 fusion proteins indicated to the left of the panels, developed with 3, 3'-diaminobenzidine (DAB) for 1 min and visualized with light microscopy at 10× magnification.
Sherwood, L. J., & Hayhurst, A. (2019). Periplasmic sdAb-APEX2 Fusions Enable Facile Visualization of Ebola, Marburg, and Mĕnglà virus Nucleoproteins, Alluding to Similar Antigenic Landscapes among Marburgvirus and Dianlovirus. Viruses, 11(4), 364.
ELISA
Figure 2 Anti-EBOV sdAb–APEX2 Fusions as ELISA Probes.
ELISA titration of sdAb-APEX2 monomers (A and E) or dimers (A2 and E2) over purified recombinant MARV or EBOV NP.
Sherwood, L. J., & Hayhurst, A. (2019). Periplasmic sdAb-APEX2 Fusions Enable Facile Visualization of Ebola, Marburg, and Mĕnglà virus Nucleoproteins, Alluding to Similar Antigenic Landscapes among Marburgvirus and Dianlovirus. Viruses, 11(4), 364.
WB
Figure 3 Visualization of MLAV NP by anti-MARV sdAb–APEX2 Fusions.
DAB developed Western blots of HEK293T cell lysates after the transient expression of the MARV, MLAV, EBOV NP genes, or a control β-galactosidase gene and probing with 10 nM of either monomeric (sdAb A and sdAb E2) or dimeric (sdAb A2 and sdAb E2) APEX2 fusion proteins. Coomassie-stained 12% gel of the lysates to show equivalent loading. Molecular weights of markers are indicated to the left in kDa.
Sherwood, L. J., & Hayhurst, A. (2019). Periplasmic sdAb-APEX2 Fusions Enable Facile Visualization of Ebola, Marburg, and Mĕnglà virus Nucleoproteins, Alluding to Similar Antigenic Landscapes among Marburgvirus and Dianlovirus. Viruses, 11(4), 364.
ELISA
Figure 4 Visualization of MLAV NP by anti-MARV sdAb–APEX2 Fusions.
ELISA titration of monomeric versus dimeric anti-MARV sdAb–APEX2 fusions over immobilized nluc–NP C-terminal domain fusions of MARV or MLAV. Curves represent an n of 2 with error bars +/− SD.
Sherwood, L. J., & Hayhurst, A. (2019). Periplasmic sdAb-APEX2 Fusions Enable Facile Visualization of Ebola, Marburg, and Mĕnglà virus Nucleoproteins, Alluding to Similar Antigenic Landscapes among Marburgvirus and Dianlovirus. Viruses, 11(4), 364.
Figure 5 Monovalent Anti-MARV sdAb Binding of Nanoluciferase MARV/MLAV–NP Fusions.
Titrations of fusions of nluc–NP C-terminal domains of either MARV or MLAV over oriented sdAb A, B or C with curves representing an n of 3 with error bars +/− SD.
Sherwood, L. J., & Hayhurst, A. (2019). Periplasmic sdAb-APEX2 Fusions Enable Facile Visualization of Ebola, Marburg, and Mĕnglà virus Nucleoproteins, Alluding to Similar Antigenic Landscapes among Marburgvirus and Dianlovirus. Viruses, 11(4), 364.
Figure 6 Locating the region of nucleoprotein (NP) bound by sdAb and establishing EC50 values.
Purified recombinant MARV or Bundibugyo (BEBOV) NP polymers were titrated over passively immobilized anti-MARV NP–sdAb, and captured NP subsequently detected with a constant amount of each phage displayed sdAb followed by anti-M13–horseradish peroxidase (HRP) conjugate.
Garza, J. A., Taylor, A. B., Sherwood, L. J., Hart, P. J., & Hayhurst, A. (2017). Unveiling a drift resistant cryptotope within marburgvirus nucleoprotein recognized by llama single-domain antibodies. Frontiers in immunology, 8, 1234.
Figure 7 Locating the region of nucleoprotein (NP) bound by sdAb and establishing EC50 values.
Fusions of sdAb–gluc were titrated over passively immobilized MARV NP polymer to determine EC50 values. Each titration was performed in duplicate wells with a negative control binding curve on BEBOV NP subtracted from each MARV curve.
Garza, J. A., Taylor, A. B., Sherwood, L. J., Hart, P. J., & Hayhurst, A. (2017). Unveiling a drift resistant cryptotope within marburgvirus nucleoprotein recognized by llama single-domain antibodies. Frontiers in immunology, 8, 1234.
Specifications
- Host Species
- Llama
- Type
- Llama VHH
- Specificity
- MARV NP
- Species Reactivity
- Lake Victoria marburgvirus (strain Musoke-80)
- Clone
- PABC-560
- Applications
- ELISA, Western Blot
Product Property
- Purity
- >95% as determined by analysis by SDS-PAGE
- Storage
- Store at -20°C for long-term storage. Avoid freeze/thaw cycles.
Applications
- Application Notes
- The antibody was validated for ELISA, Western Blot. For details, refer to Published Data.
Target
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Epitope-Specific Antibody Products
Single Domain Antibody Products
IgG Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| EPAF-0810CQ | Recombinant Mouse Anti-MARV NP Antibody (2A7) | WB, ELISA, IHC | IgG1 |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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