Mouse Anti-NHP RAD21 Recombinant Antibody (clone 1B6D1) (CAT#: VS-1024-XY429)
Aimed at detecting RAD21, this mouse monoclonal antibody shows reactivity with human, non-human primate, and rat samples. The immunogen is a purified recombinant fragment of human RAD21, providing exceptional specificity for target recognition. Suitable for Western blot (WB), immunohistochemistry (IHC), and ELISA, this antibody is a valuable resource for investigating RAD21 expression and function, enhancing the reliability of results across various experimental applications and contributing to a deeper understanding of cellular processes.
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Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & midbody.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/73277/1950_C3_2_selected.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & midbody.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/73277/1950_C3_2_selected.jpg
Figure 2 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/22065/if_selected.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/22065/if_selected.jpg
Figure 3 IHC staining of human small intestine
(Immunohistochemical staining of human small intestine shows strong nuclear positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/20044/ihc_selected.jpg
(Immunohistochemical staining of human small intestine shows strong nuclear positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/20044/ihc_selected.jpg
Figure 4 Cerebral cortex
(Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Glial cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Neuronal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/22065/48917_B_9_5.jpg
(Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Glial cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Neuronal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/22065/48917_B_9_5.jpg
Figure 5 Colon
(Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Peripheral nerve/ganglion Staining: High Intensity: Strong Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/22065/48917_A_8_3.jpg
(Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Peripheral nerve/ganglion Staining: High Intensity: Strong Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/22065/48917_A_8_3.jpg
Figure 6 Kidney
(Cells in glomeruli Staining: Low Intensity: Moderate Quantity: <25% Collecting ducts Staining: High Intensity: Strong Quantity: 75%-25% Distal tubules Staining: High Intensity: Strong Quantity: 75%-25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/22065/48917_A_7_5.jpg
(Cells in glomeruli Staining: Low Intensity: Moderate Quantity: <25% Collecting ducts Staining: High Intensity: Strong Quantity: 75%-25% Distal tubules Staining: High Intensity: Strong Quantity: 75%-25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/22065/48917_A_7_5.jpg
Figure 7 Testis
(Leydig cells Staining: High Intensity: Strong Quantity:>75% Pachytene spermatocytes Staining: High Intensity: Strong Quantity:>75% Peritubular cells Staining: Low Intensity: Moderate Quantity: <25% Preleptotene spermatocytes Staining: High Intensity: Strong Quantity:>75% Round or early spermatids Staining: High Intensity: Strong Quantity:>75% Sertoli cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Spermatogonia cells Staining: High Intensity: Strong)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/22065/48917_A_5_6.jpg
(Leydig cells Staining: High Intensity: Strong Quantity:>75% Pachytene spermatocytes Staining: High Intensity: Strong Quantity:>75% Peritubular cells Staining: Low Intensity: Moderate Quantity: <25% Preleptotene spermatocytes Staining: High Intensity: Strong Quantity:>75% Round or early spermatids Staining: High Intensity: Strong Quantity:>75% Sertoli cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Spermatogonia cells Staining: High Intensity: Strong)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/22065/48917_A_5_6.jpg
Figure 8 Lymph node
(Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/22065/48917_A_7_8.jpg
(Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/22065/48917_A_7_8.jpg
Figure 9 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000164754-RAD21
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000164754-RAD21
Specifications
- Immunogen
- Purified recombinant fragment of human RAD21
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- RAD21
- Species Reactivity
- Human, Non-human primate, Rat
- Clone
- 1B6D1
- Applications
- WB, IHC, ELISA
Product Property
- Purification
- Protein A purified
- Purity
- >95%, determined by SDS-PAGE
- Format
- Liquid
- Buffer
- PBS, pH 7.4
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Applications
- Application Notes
- WB: 1:500-1:1000, IHC: 1:200-1:800, ELISA: 1:8000
Target
- Alternative Names
- RAD21 Cohesin Complex Component; Sister Chromatid Cohesion 1; Nuclear Matrix Protein 1; SCC1 Homolog; Kleisin; NXP-1; HHR21; HR21; NXP1; Protein Involved In DNA Double-Strand Break Repair
- Gene ID
- 5885
- UniProt ID
- O60216
Recommended Products
Recommended Dilution Buffer
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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IgG Antibody Products
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IHC Kit and Antibody Products: Precision Tools for Immunohistochemistry
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-3406z | Mouse Anti-RAD21 Recombinant Antibody (clone 32D5) | WB, IP, IF, ELISA | Mouse IgM, κ |
| VS3-FY2858 | Rabbit Anti-RAD21 Recombinant Antibody (VS3-FY2858) | WB, IHC, ICC | Rabbit IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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