Human Anti-Phl p 5 Recombinant Antibody (clone 31) (CAT#: FAMAB-0012WJ)

Figure 1 Influence of the preincubation of recombinant Phl p 5 with a Phl p 5-specific IgE Fab on the IgE binding of grass pollen allergic patients.

Nitrocellulose strips containing blotted recombinant Phl p 5 (duplicates) were preincubated with Phl p 5-specific IgE Fabs (clones 31) or with Bet v 1 IgG Fabs (negative control, Co) before serum IgE from two grass pollen allergic patients was applied. Bound IgE was detected with 125I-labeled anti-human IgE monoclonal antibodies.

Steinberger, P., Kraft, D., & Valenta, R. (1996). Construction of a combinatorial IgE library from an allergic patient isolation and characterization of human IgE Fabs with specificity for the major timothy grass pollen allergen, phl p 9. Journal of Biolol Chemistry, 271(18), 10967-10972.

Figure 2 Purification of a recombinant Phl p 5-specific IgE Fab by affinity chromatography to immobilized recombinant Phl p5.

A gel containing samples of the total E. coli supernatant: Fabs (SN), the wash fraction (lane 1), and the elution fractions (lanes 2–5) of the Phl p 5 affinity column, was blotted onto nitrocellulose and Fabs were detected with a goat anti-human Fab antiserum.

Steinberger, P., Kraft, D., & Valenta, R. (1996). Construction of a combinatorial IgE library from an allergic patient isolation and characterization of human IgE Fabs with specificity for the major timothy grass pollen allergen, phl p 9. Journal of Biolol Chemistry, 271(18), 10967-10972.