Rabbit Anti-PML Recombinant Antibody (clone R02-6K4) (CAT#: VS3-XY3470)
This antibody is a recombinant rabbit antibody against PML (PML nuclear body scaffold), which functions via its association with PML-nuclear bodies (PML-NBs). It specifically reacts with human, mouse and rat PML. It has been reported for use in Western Blot, Immunohistochemistry-Paraffin, Immunoprecipitation, Flow cytometry, Immunocytochemistry or Immunofluorescence.
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Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat PML
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R02-6K4
- Applications
- WB, IHC-P, ICC, IF, IP, FC
- Related Disease
- Acute Promyelocytic Leukemia; Rabies
Product Property
- Clonality
- Monoclonal
- Aggregation
- < 5%
- Format
- Liquid
- Buffer
- PBS (pH 7.4), 150 mM NaCl and 50% glycerol
- Preservative
- 0.02% sodium azide
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- WB: 1/500-1/1000
IHC: 1/50-1/100
IF: 1/50-1/200
IP: 1/20
FC: 1/50-1/100
Target
- Alternative Names
- MYL; RNF71; PP8675; TRIM19
- Gene ID
- 5371
- UniProt ID
- P29590
- Long Name
- PML nuclear body scaffold
- Cellular Localization
- Nucleus, nucleoplasm
- Post Translation Modifications
- Ubiquitinated; mediated by RNF4, RNF111, UHRF1, UBE3A/E6AP, BCR(KLHL20) E3 ubiquitin ligase complex E3 ligase complex, SIAH1 or SIAH2 and leading to subsequent proteasomal degradation (PubMed:18408734, 21840486, 22033920).
Ubiquitination by BCR(KLHL20) E3 ubiquitin ligase complex E3 ligase complex requires CDK1/2-mediated phosphorylation at Ser-518 which in turn is recognized by prolyl-isopeptidase PIN1 and PIN1-catalyzed isomerization further potentiates PML interaction with KLHL20 (PubMed:21840486, 22033920).
'Lys-6'-, 'Lys-11'-, 'Lys-48'- and 'Lys-63'-linked polyubiquitination by RNF4 is polysumoylation-dependent (PubMed:18408734).
Ubiquitination by RNF111 is polysumoylation-dependent.
- Protein Refseq
- NP_150241.2
- Function
- Functions via its association with PML-nuclear bodies (PML-NBs) in a wide range of important cellular processes, including tumor suppression, transcriptional regulation, apoptosis, senescence, DNA damage response, and viral defense mechanisms.
Acts as the scaffold of PML-NBs allowing other proteins to shuttle in and out, a process which is regulated by SUMO-mediated modifications and interactions.
Inhibits EIF4E-mediated mRNA nuclear export by reducing EIF4E affinity for the 5' 7-methylguanosine (m7G) cap of target mRNAs.
Recommended Products
Isotype Control
Recommended Dilution Buffer
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2034z | Mouse Anti-PML Recombinant Antibody (clone 29B8) | WB, ELISA, IF, IHC, IP | Mouse IgG2b, κ |
| MOB-1287CT | Recombinant Mouse anti-Human PML Monoclonal antibody (EML1510) | WB | |
| VS3-FY1157 | Recombinant Rabbit Anti-PML Antibody (clone R04-2B3) | WB, IHC-Fr, IHC-P, ICC, IF, IP | Rabbit IgG |
| VS3-WK2039 | Rabbit Anti-PML (pS518) Recombinant Antibody (clone R01-3D1) | WB, IHC-P | Rabbit IgG |
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For Research Use Only. Not For Clinical Use.
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