Rabbit Anti-PNMT Recombinant Antibody (clone R17-1Y-5)
CAT#: VS3-FY2181
Clone R17-1Y-5 is a rabbit recombinant antibody that is specific for human PNMT protein, a calculated weight of 31 kDa. It cross-reacts with mouse.
The following recommendations serve as a starting point for assay optimization. The actual working concentration may vary and should be determined by the user. The R17-1Y-5 is recommended for detection of target protein by western blotting (dilution range 1:500-1:1000), immunofluorescence (dilution range 1:50-1:200).
Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human and Mouse PNMT
- Species Reactivity
- Human, Mouse
- Clone
- R17-1Y-5
- Applications
- Western Blot, Immunofluorescence
- Conjugate
- Unconjugated
- MW
- The calculated weight of the target protein is approximately 31 kDa, while the observed molecular weight stands at 30kDa.
- Related Disease
- Von Hippel-Lindau Syndrome and Multiple Endocrine Neoplasia
Product Property
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS (pH 7.4), 150 mM NaCl, 50% glycerol
- Preservative
- 0.02% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunofluorescence: 1/50-1/200
Target
- Alternative Names
- PENT
- Gene ID
- 5409
- UniProt ID
- P11086
- Sequence Similarities
- Belongs to the class I-like SAM-binding methyltransferase superfamily. NNMT/PNMT/TEMT family.
- Cellular Localization
- Cytosol
- Post Translation Modifications
- Modification sites at PhosphoSitePlus (P11086)
Modification sites at neXtProt (NX_P11086)
- Protein Refseq
- XP_011523211.1; XP_054172493.1; NP_002677.1
- Function
- Catalyzes the transmethylation of nonepinephrine (noradrenaline) to form epinephrine (adrenaline), using S-adenosyl-L-methionine as the methyl donor.
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "PNMT"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-3507z | Mouse Anti-PNMT Recombinant Antibody (clone 16F1) | WB, ELISA, ICC, IF | Mouse IgG2a, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2768 | Hi-Affi™ Recombinant Rabbit Anti-PNMT Monoclonal Antibody (DS2768AB) | WB, ICC, IF | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC106 | AbPlus™ Anti-PNMT Magnetic Beads (VS-0724-YC106) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY398 | Mouse Anti-NHP PNMT Recombinant Antibody (clone OTI1D2) | WB, IHC, IF | Mouse IgG2b |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY5528 | Anti-PNMT Immunohistochemistry Kit | IHC |
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