Rabbit Anti-PRP4K Recombinant Antibody (clone 2506W44) (CAT#: VS-0625-FY66)

• Immunogen
• A synthetic peptide designed to encompass residues surrounding Lys659 in human PRP4K protein.

• Host Species
• Rabbit

• Type
• Rabbit IgG

• Specificity
• This Rabbit monoclonal antibody (mAb) recognizes endogenous levels of total PRP4K protein.

• Species Reactivity
• Human, Rat, Monkey

• Clone
• 2506W44

• Applications
• Western Blot, Immunoprecipitation

• Purification
• Purified with Protein A or G affinity chromatography

• Purity
• >90% as determined by SDS-PAGE

• Concentration
• Please refer to the vial label for the specific concentration.

• Storage
• Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

• Shipping
• Ice packs

• Application Notes
• Western Blot: 1:1000
  Immunoprecipitation: 1:50
  Optimal dilutions for assays should be determined by the user.

• Alternative Names
• KIAA0536, Prp4B, Prp4, PR4H, Serine/Threonine-Protein Kinase PRP4 Homolog, PRP4 Pre-MRNA-Processing Factor 4 Homolog, Pre-MRNA Processing Factor 4B, PRP4 Kinase, EC 2.7.11.1

• Gene ID
• 8899

• UniProt ID
• Q13523

• Long Name
• Pre-MRNA Processing Factor Kinase PRP4K

• Sequence Similarities
• Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family.

• Cellular Localization
• Nucleus {ECO:0000269 PubMed:12077342}
  Chromosome, centromere, kinetochore

• Post Translation Modifications
• Phosphorylated by CLK1

• Protein Refseq
• Phosphorylated; by; CLK1

• Function
• Serine/threonine kinase involved in spliceosomal assembly as well as mitosis and signaling regulation (PubMed:10799319, 12077342, 17513757, 17998396).
  Connects chromatin mediated regulation of transcription and pre-mRNA splicing (PubMed:12077342).
  During spliceosomal assembly, interacts with and phosphorylates PRPF6 and PRPF31, components of the U4/U6-U5 tri-small nuclear ribonucleoprotein (snRNP), to facilitate the formation of the spliceosome B complex.
  Plays a role in regulating transcription and the spindle assembly checkpoint (SAC).

Secondary Antibody

• 2ND-054CQ
• Goat Anti-Rabbit IgG Antibody, R-PE

• 2ND-055CQ
• Chicken Anti-Rabbit IgG (H&L) Antibody

• 2ND-057CQ
• Donkey Anti-Rabbit IgG (H&L) Antibody

• 2ND-059CQ
• Goat Anti-Rabbit IgG (H&L) Antibody

Isotype Control

• MOB-208CQ
• Rabbit IgG Isotype Control Antibody