Rabbit Anti-RPS8 Recombinant Antibody (clone R05-5I8)

CAT#: VS3-FY2082

This R05-5I8 recombinant monoclonal antibody targets for human RPS8. It cross-reacts with mouse, rat, hamster. It is suitable for use in a variety of assays, including WB, ICC/IF, IP. The specificity of the RPS8 antibody was confirmed by western blot analysis of RPS8 in Raw264.7, PC-12 lysates.

Tested Data
Figure 1 Anti-RPS8 Antibody (VS3-FY2082) in WB.

Specifications

  • Immunogen
  • Recombinant human RPS8 protein
  • Host Species
  • Rabbit
  • Type
  • Rabbit IgG
  • Specificity
  • Human, Mouse, Rat, Hamster RPS8
  • Species Reactivity
  • Human, Mouse, Rat, Hamster
  • Clone
  • R05-5I8
  • Applications
  • Western Blot, Immunofluorescence, Immunoprecipitation
  • MW
  • The calculated weight of the target protein is approximately 24 kDa, while the observed molecular weight stands at 24 kDa.
  • Related Disease
  • Theileriasis and Babesiosis

Product Property

  • Purification
  • Affinity Purified
  • Format
  • Liquid
  • Concentration
  • Please refer to the vial label for the specific concentration.
  • Buffer
  • 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% glycerol, 0.05% BSA
  • Preservative
  • 0.01% Sodium azide
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

  • Application Notes
  • Western Blot: 1/500-1/1000
    Immunofluorescence: 1/50-1/200
    Immunoprecipitation: 1/20

Target

  • Alternative Names
  • Ribosomal Protein S8; Small Ribosomal Subunit Protein ES8; 40S Ribosomal Protein S8; OK/SW-Cl.83; S8
  • Sequence Similarities
  • Belongs to the eukaryotic ribosomal protein eS8 family.
  • Cellular Localization
  • Cytoplasm, Membrane, Nucleus
  • Post Translation Modifications
  • Ubiquitination at Lys37, Lys98, Lys157, Lys170, and Lys193 (NX_P62241 [NX_P62241-1])
    Modification sites at PhosphoSitePlus (P62241)
    Glycosylation from GlyGen (P62241) 1 site, 1 O-linked glycan (1 site)
  • Function
  • The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell.
    Part of the small subunit (SSU) processome, first precursor of the small eukaryotic ribosomal subunit. During the assembly of the SSU processome in the nucleolus, many ribosome biogenesis factors, an RNA chaperone and ribosomal proteins associate with the nascent pre-rRNA and work in concert to generate RNA folding, modifications, rearrangements and cleavage as well as targeted degradation of pre-ribosomal RNA by the RNA exosome.
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Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

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Protocol & Troubleshooting

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