Anti-RPSA (clone BV-27) Recombinant Antibody Coupled Liposome (VS-1024-FY2) (CAT#: VS-1024-FY2)

• Potential Clinical Applications
• The antibody is covalently attached to the surface of the liposome, which is coated with doxorubicin via a disulfide bond, thereby exhibiting the capacity to selectively target neoplastic cells.

• Immunoliposomes Method
• The liposome formulation containing encapsulated doxorubicin was combined with an IgM solution and incubated at 37 °C for 1 to 2 hours. Following this incubation, the mixture was treated with cysteine at a concentration of 0.83 mg/mL for 30 minutes to effectively block any unreacted maleimide groups on the liposome surfaces. The resulting chemoimmunoliposome suspension was then loaded onto a Sepharose CL-4B column and eluted using a solution of 2.5% mannitol in phosphate-buffered saline. The chemoimmunoliposomes were collected in the void volume fraction.

• Clone
• BV-27

• Antibody Type
• IgG2a

• Antibody Host
• Mouse

• Antibody Reactivity
• Human

• Antibody Description
• This is a mouse monoclonal antibody that is a specific designed to target the human laminin receptor. This antibody was developed through a series of immunization and hybridoma techniques aimed at producing a robust immune response against a synthetic peptide derived from the laminin receptor, known as LR-1 (Leu-23-His).

• Storage
• See in the COA

• Storage Shelf Time
• See in the COA

• Target
• RPSA

• Introduction
• Laminins, a family of extracellular matrix glycoproteins, are the major noncollagenous constituent of basement membranes. They have been implicated in a wide variety of biological processes including cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis. Many of the effects of laminin are mediated through interactions with cell surface receptors. These receptors include members of the integrin family, as well as non-integrin laminin-binding proteins. This gene encodes a high-affinity, non-integrin family, laminin receptor 1. This receptor has been variously called 67 kD laminin receptor, 37 kD laminin receptor precursor (37LRP) and p40 ribosome-associated protein. The amino acid sequence of laminin receptor 1 is highly conserved through evolution, suggesting a key biological function. It has been observed that the level of the laminin receptor transcript is higher in colon carcinoma tissue and lung cancer cell line than their normal counterparts. Also, there is a correlation between the upregulation of this polypeptide in cancer cells and their invasive and metastatic phenotype. Multiple copies of this gene exist, however, most of them are pseudogenes thought to have arisen from retropositional events. Two alternatively spliced transcript variants encoding the same protein have been found for this gene.

• Alternative Names
• Ribosomal Protein SA; Laminin Receptor 1 (67kD, Ribosomal Protein SA); Multidrug Resistance-Associated Protein MGr1-Ag; Colon Carcinoma Laminin-Binding Protein; Laminin-Binding Protein Precursor P40; Small Ribosomal Subunit Protein US2; 37/67 KDa Laminin Receptor; 7 KDa Laminin Receptor; NEM/1CHD4; LBP/P40; LRP/LR; LAMR1; 37LRP

• Full Name
• Ribosomal Protein SA

• Gene ID
• 3921

• UniProt ID
• P08865

• Sequence Similarities
• Belongs to the universal ribosomal protein us2 family.

• Cellular Localization
• Cell membrane, Cytoplasm, Nucleus

• Post Translation Modifications
• Acylated

• Protein Refseq
• NP_001291217.1; NP_002286.2

• Function
• Required for the assembly and/or stability of the 40S ribosomal subunit.
  Required for the processing of the 20S rRNA-precursor to mature 18S rRNA in a late step of the maturation of 40S ribosomal subunits.
  Also functions as a cell surface receptor for laminin.
  Plays a role in cell adhesion to the basement membrane and in the consequent activation of signaling transduction pathways.
  May play a role in cell fate determination and tissue morphogenesis.

• HPAB-0132-WJ
• Mouse Anti-RPSA Recombinant Antibody (clone BV-27)

• HPAB-0132-WJ-S(P)
• Mouse Anti-RPSA Recombinant Antibody (clone BV-27); scFv Fragment

• HPAB-0132-WJ-F(E)
• Mouse Anti-RPSA Recombinant Antibody (clone BV-27); Fab Fragment

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