Chicken Anti-SARS-CoV S Recombinant Antibody (clone 4S3) (CAT#: VS-0323-CJ48)
This chicken anti-SARS-CoV S antibody (clone 4S3) is a product that recognizes SARS-CoV S proteins.
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
Figure 1 Binding activity of randomly chosen phages from library 4S after the fourth panning cycle.
Fifteen phage clones (including VS-0323-CJ48) were amplified and prepared to test their binding to SARS-CoV-infected Vero E6 cell lysates in an ELISA test.
Specifications
- Immunogen
- Recombinant S proteins
- Host Species
- Chicken
- Type
- Chicken IgY
- Specificity
- SARS-CoV S proteins
- Species Reactivity
- SARS-CoV
- Clone
- 4S3
- Conjugate
- Unconjugated
- Related Disease
- Severe acute respiratory syndrome (SARS)
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- PBS
- Storage
- Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The Phage clone of this antibody exhibited significant binding activity (OD between 0.7 and 1.2) to SARS-CoV S protein.
Target
- Alternative Names
- E2
- Gene ID
- 1489668
- UniProt ID
- P59594
- Sequence Similarities
- Belongs to the betacoronaviruses spike protein family.
- Cellular Localization
- Host cell membrane; Host membrane; Membrane; Viral envelope protein; Virion
- Post Translation Modifications
- The cytoplasmic Cys-rich domain is palmitoylated. Spike glycoprotein is digested by cathepsin CTSL within endosomes.
Specific enzymatic cleavages in vivo yield mature proteins. The precursor is processed into S1 and S2 by host cell furin or another cellular protease to yield the mature S1 and S2 proteins. Additionally, a second cleavage leads to the release of a fusion peptide after viral attachment to host cell receptor.
The cytoplasmic Cys-rich domain is palmitoylated. Spike glycoprotein is digested within host endosomes.
- Protein Refseq
- NP_828851.1
- Function
- Spike glycoprotein: May down-regulate host tetherin (BST2) by lysosomal degradation, thereby counteracting its antiviral activity.
Spike protein S1: Attaches the virion to the cell membrane by interacting with host receptor, initiating the infection. Binding to human ACE2 and CLEC4M/DC-SIGNR receptors and internalization of the virus into the endosomes of the host cell induces conformational changes in the S glycoprotein. Proteolysis by cathepsin CTSL may unmask the fusion peptide of S2 and activate membrane fusion within endosomes.
Spike protein S2: Mediates fusion of the virion and cellular membranes by acting as a class I viral fusion protein. Under the current model, the protein has at least three conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state. During viral and target cell membrane fusion, the coiled coil regions (heptad repeats) assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain. The formation of this structure appears to drive apposition and subsequent fusion of viral and target cell membranes.
Spike protein S2': Acts as a viral fusion peptide which is unmasked following S2 cleavage occurring upon virus endocytosis.
Recommended Products
Isotype Control
Secondary Antibody
Recommended Dilution Buffer
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "SARS-CoV S"
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CAT | Product Name | Application | Type |
---|---|---|---|
PABW-114 | Recombinant Mouse Anti-SARS-CoV S Antibody ( F26G19) | WB, ELISA | IgG |
MRO-1345CQ | Recombinant Mouse Anti-SARS-CoV E2 Antibody (4A6C9) | ELISA, WB | Mouse antibody |
HPAB-1337WJ | Human Anti-SARS-CoV S Recombinant Antibody (HPAB-1337WJ) | ELISA, FC, WB, IHC, IP | Human IgG2 |
MRO-0010YJ | Human Anti-SARS-CoV S Recombinant Antibody (clone CBS007) | WB, ELISA, IF, Neut | Human IgG1, κ |
VS-0323-CJ47 | Chicken Anti-SARS-CoV S Recombinant Antibody (clone 4S1) | ELISA, WB, IF | Chicken IgY |
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