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  • Mouse Anti-SDCBP Recombinant Antibody (VS7-0425-WR834)

Mouse Anti-SDCBP Recombinant Antibody (VS7-0425-WR834) (CAT#: VS7-0425-WR834)

This product is a mouse IgG antibody that binds to human SDCBP. The antibody can be used for WB assay. It cross reacts with rat, dog, and pig.

Specific Inquiry
  • Size:
  • Conjugation:
  • Endotoxin:
  • Purity:
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  • Modalities:
  • Gene Expression
  • Datasheet
  • MSDS
  • COA
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line A-431
Figure 1 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm, nuclear membrane & cytosol.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/23840/if_selected.jpg
Figure 2 IHC staining of human prostate
Figure 2 IHC staining of human prostate
(Immunohistochemical staining of human prostate shows strong positivity in peripheral nerves.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/23840/ihc_selected.jpg
Figure 3 IHC staining of human vulva/anal skin
Figure 3 IHC staining of human vulva/anal skin
(Immunohistochemical staining of human vulva/anal skin shows distinct cytoplasmic positivity in melanocytes.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/12245/ihc_selected.jpg
Figure 4 IF staining of human cell line U-2 OS
Figure 4 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm, nuclear membrane & cytosol.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/23840/241_E2_1_red_green.jpg
Figure 5 IF staining of human cell line U-251 MG
Figure 5 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm, nuclear membrane & cytosol.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/23840/239_E2_1_red_green.jpg
Normal Tissue
Figure 6 Cerebral cortex
Figure 6 Cerebral cortex
(Neuronal cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/12245/31141_B_8_5.jpg
Figure 7 Colon
Figure 7 Colon
(Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Glandular cells
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
Peripheral nerve/ganglion
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/12245/31141_A_7_3.jpg
Figure 8 Skin
Figure 8 Skin
(Epidermal cells
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/12245/31141_B_5_8.jpg
Figure 9 Lymph node
Figure 9 Lymph node
(Germinal center cells
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/12245/31141_A_9_8.jpg
RNA Expression
Figure 10 RNA cell line category: Cell line enhanced (HHSteC)
Figure 10 RNA cell line category: Cell line enhanced (HHSteC)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000137575-SDCBP

Specifications

  • Immunogen
  • Recombinant human SDCBP fragment expressed in E. Coli.
  • Host Species
  • Mouse
  • Type
  • Mouse IgG
  • Specificity
  • Human SDCBP
  • Species Reactivity
  • Human, Rat, Dog, Pig
  • Molecule Class
  • Pdz-Domain-Containing Protein
  • Applications
  • WB
  • Conjugate
  • Unconjugated

Product Property

  • Clonality
  • Monoclonal
  • Purity
  • >95% as determined by SDS-PAGE and SEC-HPLC analysis, Ascitic fluid
  • Format
  • Liquid
  • Concentration
  • 1 mg/ml
  • Buffer
  • 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl)
  • Preservative
  • 0.2% sodium azide
  • Storage
  • Centrifuge briefly prior to opening vial. Aliquot and store at -20°C for one year. Avoid repeated freeze/thaw cycles.
  • Shipping
  • Ice packs

Applications

  • Application Notes
  • Western Blot: 1:1000 - 1:2000

Target

  • Alternative Names
  • ST1; MDA9; SYCL; MDA-9; TACIP18
  • Long Name
  • Syndecan Binding Protein
  • Cellular Localization
  • Cell junction; Cell membrane; Cytoplasm; Cytoskeleton; Endoplasmic reticulum; Membrane; Nucleus; Secreted
  • Post Translation Modifications
  • Acetylation; Phosphoprotein
  • Function
  • Multifunctional adapter protein involved in diverse array of functions including trafficking of transmembrane proteins, neuro and immunomodulation, exosome biogenesis, and tumorigenesis (PubMed:26291527).; Positively regulates TGFB1-mediated SMAD2/3 activation and TGFB1-induced epithelial-to-mesenchymal transition (EMT) and cell migration in various cell types. May increase TGFB1 signaling by enhancing cell-surface expression of TGFR1 by preventing the interaction between TGFR1 and CAV1 and subsequent CAV1-dependent internalization and degradation of TGFR1 (PubMed:25893292).; In concert with SDC1/4 and PDCD6IP, regulates exosome biogenesis (PubMed:22660413).; Regulates migration, growth, proliferation, and cell cycle progression in a variety of cancer types (PubMed:26539120).; In adherens junctions may function to couple syndecans to cytoskeletal proteins or signaling components. Seems to couple transcription factor SOX4 to the IL-5 receptor (IL5RA) (PubMed:11498591).; May also play a role in vesicular trafficking (PubMed:11179419).; Seems to be required for the targeting of TGFA to the cell surface in the early secretory pathway (PubMed:10230395).

Recommended Products

Isotype Control

Secondary Antibody

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

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CAT Product Name Application Type
NAB-1089-VHH Recombinant Anti-human SDCBP VHH Single Domain Antibody WB, IP, ICC, FC, FA Llama VHH

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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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