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  • Mouse Anti-ST8Sia4 Recombinant Antibody (clone 3175)

Mouse Anti-ST8Sia4 Recombinant Antibody (clone 3175) (CAT#: VS-0723-CJ35)

This mouse antibody 3175 specifically binds to human and hamster ST8Sia4. The immunogen was the purified hamster ST8Sia4 protein composed of amino acids residues Arg27 to Gln359. This antibody has been tested for use in enzyme-linked immunosorbent assay, flow cytometry, immunofluorescence, immunoblotting and inhibition assays.


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Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line A549
Figure 1 IF staining of human cell line A549
(Immunofluorescent staining of human cell line A549 shows localization to the Golgi apparatus.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/53995/1125_C4_2_selected.jpg
Figure 2 IHC staining of human kidney
Figure 2 IHC staining of human kidney
(Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in cells in tubules.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/51126/ihc_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Figure 3 Cerebral cortex
(Neuronal cells
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Neuropil
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/51126/116425_B_7_5.jpg
Figure 4 Colon
Figure 4 Colon
(Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Glandular cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Peripheral nerve/ganglion
Staining: Low
Intensity: Weak
Quantity:>75%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/51126/116425_A_9_3.jpg
Figure 5 Liver
Figure 5 Liver
(Hepatocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/51126/116425_A_7_4.jpg
Figure 6 Kidney
Figure 6 Kidney
(Cells in tubules
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/51126/116425_A_8_5.jpg
Figure 7 Testis
Figure 7 Testis
(Leydig cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/51126/116425_A_6_6.jpg
Figure 8 Lymph node
Figure 8 Lymph node
(Germinal center cells
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/51126/116425_A_8_8.jpg
Figure 9 Heart
Figure 9 Heart
(Cardiomyocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/51126/116425_B_6_6.jpg
RNA Expression
Figure 10 RNA cell line category: Cell line enhanced (AF22, HDLM-2, Karpas-707, NB-4, RPMI-8226)
Figure 10 RNA cell line category: Cell line enhanced (AF22, HDLM-2, Karpas-707, NB-4, RPMI-8226)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000113532-ST8SIA4/subcellular

Specifications

  • Immunogen
  • Protein A-polyST fusion proteins which compassed Arg27 to Gln359 of hamster ST8SiaIV
  • Host Species
  • Human
  • Type
  • Mouse IgG
  • Specificity
  • Human ST8Sia4
  • Species Reactivity
  • Human, Hamster
  • Clone
  • 3175
  • Applications
  • ELISA, FC, IF, IB, Inhib
  • Conjugate
  • Unconjugated
  • Related Disease
  • Viral infections; Brain diseases; Cancer

Product Property

  • Purification
  • Protein G purified
  • Purity
  • >95%
  • Buffer
  • PBS
  • Storage
  • Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.

Applications

  • Application Notes
  • Immobilized FLAG-HA tagged ST8SiaIV was incubated with the mAb 3175 in ELISA assay.

Target

  • Alternative Names
  • ST8SiaIV

Recommended Products

Isotype Control

Secondary Antibody

Recommended Dilution Buffer

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

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See other products for "ST8SIA4"

Recombinant Antibody

CAT Product Name Application Type
MOB-2228z Mouse Anti-ST8SIA4 Recombinant Antibody (clone 6F6) WB, ELISA Mouse IgG2a, κ

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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