Mouse Anti-Tn Antigen Recombinant Antibody (clone MLS128) (CAT#: HPAB-0319CQ)

Figure 1 Specificity of VVL and antibodies on large trimer amino acid glycopeptide library, printed on microarray slides.

The library was divided into five groups according to the position of glycosylated (GalNAc) serine (S*) and threonine (T*) in relation to other amino acids (X). VVL, scFv sH1 and G2-D11, mouse monoclonal GOD3-2C4 and MLS128 antibodies were tested in all combinations. Each dot represents a unique sub-library member. Median signals for each group of combinations are marked with a blue line and a red circle is highlighting amino acids (X) affecting binding of the reagents. (A) Group 1 (XT*T*). (B) Group 2 (T*T*X). (C) Group 3 (XT*S*). (D) Group 4 (S*T*X). (E) Group 5a (GT*X3, PT*X3, WT*X3, IT*X3, LT*X3, MT*X3, FT*X3, YT*X3 and VT*X3), where G2-D11 bound to FT*R, WT*K, YT*W and YT*A, and GOD3-2C4 bound to VT*N, VT*M, VT*W, WT*C, WT*D, WT*E, WT*I, WT*M, WT*N, YT*F, YT*M and YT*R. (F) Group 5b (RT*X3, HT*X3, KT*X3, DT*X3, ET*X3, ST*X3, TT*X3, NT*X3 and QT*X3), where GOD3-2C4 bound to HT*R. This figure is available in black and white in print and in colour at Glycobiology online.

Persson, N., Stuhr-Hansen, N., Risinger, C., Mereiter, S., Polónia, A., Polom, K., ... & Danielsson, L. (2017). Epitope mapping of a new anti-Tn antibody detecting gastric cancer cells. Glycobiology, 27(7), 635-645.

Figure 2 Specificity evaluation of Tn-binding antibodies on glycopeptide microarray.

Glycopeptides were printed on a microarray chip. MLS128 were titrated from 20 μg/mL in a series of 10, 5, 1, 0.5, 0.1 and 0.05 μg/mL.

Persson, N., Stuhr-Hansen, N., Risinger, C., Mereiter, S., Polónia, A., Polom, K., ... & Danielsson, L. (2017). Epitope mapping of a new anti-Tn antibody detecting gastric cancer cells. Glycobiology, 27(7), 635-645.